2019 Fiscal Year Annual Research Report
Development of a novel technique for direct analysis of single lipid droplets in mammalian cells by nanoESI-MS
| Project/Area Number |
18K14247
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| Research Institution | Hokkaido University |
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Principal Investigator |
趙 瑤瑤 北海道大学, 保健科学研究院, 助教 (50800702)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Keywords | single lipid droplet / Mass spectrometry / solvent microextraction |
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| Outline of Annual Research Achievements |
Diets rich in long-chain fatty acids (LCFAs, 14 or more carbon atoms) promote lipid accumulation and insulin resistance, whereas medium-chain fatty acids (MCFAs) are associated with increased oxidative metabolism and reduced adiposity, with few deleterious effects on insulin action. Lipid droplets (LDs) emerge as an important intracellular organelle relevant for lipid homeostasis and development of metabolic diseases. However, the relationship between MCFAs and the LDs composition has been poorly investigated. This study aims to investigate the effect of medium-chain fatty acid (MCFA) on triglyceride (TG) profile of LDs in HepG2 cells.We have developed a novel technique termed in-tip solvent microextraction mass spectrometry (ITSME-MS) to separate phosphatidylcholines and triglycerides in a single cellular lipid droplet. The present results suggests that MCFAs can be involved in TG synthesis in HepG2 cells in the presence of LCFAs. This finding is different to recent studies, which considered that MCFA undergoes preferential oxidation and behaves more like glucose rather than fat. This research might help us to find a way to manipulate LD metabolism in the human liver using dietary MCFAs.
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