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2023 Fiscal Year Research-status Report

Understanding the specificity of interspecies interactions between algae and bacteria in red snow by comparative proteomics and environmental monitoring

Research Project

Project/Area Number 18K14368
Research InstitutionHokkaido University

Principal Investigator

寺島 美亜  北海道大学, 低温科学研究所, 客員教授 (00756801)

Project Period (FY) 2021-11-01 – 2025-03-31
KeywordsPsychrophiles / Microalgae / Algae bacteria syntrophy / Snow microbes / Algae biotechnology
Outline of Annual Research Achievements

Green-colored arctic snow samples that contained microalgae and bacteria were obtained for sequencing analyses and strain isolation. 16S rRNA and 18S rRNA sequencing revealed that the snow samples are dominated by chlorophyte algae species and bacteria stemming from the class Bacteroidia and Gammaproteobacteria. From these snow samples, over 20+ bacterial strains have been isolated and identified via Nanopore sequencing, with several potentially novel species being identified. Additionally, metagenome sequencing of these samples are completed and are currently being analyzed.
Additionally, datamining of the transcriptome of a cryophilic snow alga has been started. Preliminary findings suggest potentially biotechnologically interesting cold-adapted enzymes unique to microorganisms found in the snow environment.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

Progress has been made due to isolation of novel strains and the analysis of the microbial community of snow samples. The metagenome data is expected to provide additional novel findings. Transcriptome analysis was attempted, but due to the bad quality of the samples, has so far not been successful.

Strategy for Future Research Activity

Data analysis for the metagenome sequencing and comparison of this data to the 16S rRNA and 18S rRNA data is planned. Additionally, further characterization of isolated bacterial strain is planned. This will provide valuable insights into the physiology of these snow algal strains that have an important impact in alpine and arctic ecosystems.

Causes of Carryover

The planned sequencing experiments and data analyses could not be fully completed this year, so we have transferred the budget for the next year.
Next year, we are planning on using the remaining budget to continue the project, particularly in the metagenome and transcriptome analyses. Additionally, cultivation experiments will be conducted on the isolated bacterial strains.

  • Research Products

    (1 results)

All Other

All Int'l Joint Research (1 results)

  • [Int'l Joint Research] Berliner Hochschule fuer Technik(ドイツ)

    • Country Name
      GERMANY
    • Counterpart Institution
      Berliner Hochschule fuer Technik

URL: 

Published: 2024-12-25  

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