Visualization of uncultured microorganisms by using a novel imaging method and challenge of single cell genomics.

2021 Fiscal Year Final Research Report

• Project/Area Number: 18K14514
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 40030:Aquatic bioproduction science-related
• Research Institution: Matsue National College of Technology
• Principal Investigator: Yamaguchi Tsuyoshi 松江工業高等専門学校, 環境・建設工学科, 准教授 (30759832)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Keywords: click chemistry / 高感度FISH法 / 機能遺伝子

Outline of Final Research Achievements

In this study, we focused on single cell genomics, which can reveal the ecology of uncultured microorganism, and we developed a novel visualization technique for sorting of uncultured microorganism. Hybridization chain reaction-fluorescence in situ hybridization (HCR-FISH) was combined for detection of functional genes in microorganisms. When this method was applied to E.coli encoding partial functional genes, signal was obtained from E.coli by investigated reaction time and concertation of reagent of click chemistry. Moreover, some signal was obtained from microorganisms in bioreactor.

Free Research Field

環境微生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では, 未培養微生物を視覚的に検出する手法開発を行った. この手法開発を用いて視覚的検出によって, 微生物回収が容易になり, 新たな微生物資源の発掘や微生物生態の解明につながると考えている. 社会的意義としても, 新たな微生物資源を発掘することで, 新規の水処理装置や環境改善に利用することができることから貢献度は高いと考えている.