The generation of a novel mouse artificial chromosome carrying pseudo-autosomal region, and analysis of its stability in germline.

2021 Fiscal Year Final Research Report

• Project/Area Number: 18K14617
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42040:Laboratory animal science-related
• Research Institution: Tottori University
• Principal Investigator: Yoshimura Yuki 鳥取大学, 医学部, 助教 (50771242)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Keywords: 遺伝子改変動物 / 擬似常染色体領域 / 人工染色体

Outline of Final Research Achievements

Mouse artificial chromosomes (MACs) are unique vectors in which the gene region of a mouse chromosome is removed to allow the insertion of transgenes. Although many transchromosomic (Tc) mice that retain MAC have been generated, the low rates of germline transmission of MAC derived from male Tc mice have been reported, and this cause remains unknown. Pseudoautosomal regions (PARs) are located at the ends of sex chromosomes and have high homology between X and Y chromosomes. The X and Y chromosomes can be paired between each PAR region. In order to find clues to improve the germline transmission rate of MAC, I attempted to construct a novel mouse artificial chromosome carrying the PAR region, and trace it in meiosis.

Free Research Field

実験動物学

Academic Significance and Societal Importance of the Research Achievements

人工染色体ベクターは100kbからMb単位に及ぶ遺伝子でさえ搭載することが可能であり、他にはないユニークな特徴を持つ。人工染色体ベクターを用いて、完全ヒト抗体産生マウスなど従来法では作製不可能であったモデル動物が数多く作製されており、疾患の原因解明や創薬開発に貢献している。一方で、Tcマウスの繁殖性には課題があり、より汎用的に利用されるためにはこの課題を克服する必要がある。本研究で得られた成果は、この課題克服に貢献するものである。