2020 Fiscal Year Annual Research Report
Dynamic Character of the Ubiquitin System
| Project/Area Number |
18K14665
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| Research Institution | Kyoto University |
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Principal Investigator |
Walinda Erik 京都大学, 医学研究科, 助教 (80782391)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | ubiquitin / ubiquitin binding / UBA domain / K48-linked chains / K63-linked chains / M1-linked chains / millisecond dynamics / solution NMR |
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| Outline of Annual Research Achievements |
Research has proceeded smoothly overall and we have been able to publish several results of this study. In particular, the molecular dynamics simulations and NMR experiments of ubiquitin chains have produced interesting results that well agree with observations by other researchers worldwide in other fields (e.g., X-ray crystallography).
Specifically, distinct ubiquitin chain conformations of the same molecule (e.g., K48-linked Ub2) are reported by different researchers using different crystallization conditions. All of these forms however, have been found to be on the same trajectory in phase-space as sampled by molecular dynamics. The main result of this project is summarized in our 2021 Biochemistry paper. However, several aspects of this study (especially for chains other than the K48-linked ubiquitin chain) are yet to be published.
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