Establishment of new human pluripotent stem cell with unbiased differentiation potency.

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K14714
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 44020:Developmental biology-related
• Research Institution: Kyoto University
• Principal Investigator: Nakamura Tomonori 京都大学, 白眉センター, 特定准教授 (90648429)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: カニクイザル / 多能性幹細胞 / scRNA-seq

Outline of Final Research Achievements

Pluripotent stem cells (PSCs) are cells that can proliferate indefinitely while retaining the ability to contribute to whole adult cells. In this study, we attempted to establish a new culture system that eliminates the differentiation bias seen in human PSCs by mimicking the environment in vivo. Since the target in vivo pluripotent cells correspond immediately after implantation, we aimed to analyze the traits of cynomolgus monkey implantation embryos using scRNA-seq, examine the culture conditions using the monkey PSC, and reproduce them in human PSC. In this process, we have established a new robust and stable culture system for monkey PSC, which has been unstable, and surprisingly discovered a universal problem in scRNA-seq analysis, and found a solution.

Free Research Field

発生、幹細胞生物学

Academic Significance and Societal Importance of the Research Achievements

ヒトiPSCが樹立されて以来、モデル動物としての非ヒト霊長類が見直されてきている。また、霊長類に関わらず昨今の分子生物学ではscRNA-seqを筆頭に単一細胞レベルの研究領域に突入している。これらの状況を鑑みると本研究成果はこれからの時代の基礎となる知見や技術になり、学術的意義は高いと考える。今後本研究の成果をもとに有意義な研究を展開していきたい。