Overview of the regulation of skeletal muscle metabolism based on comprehensive molecular quantification

2022 Fiscal Year Final Research Report

• Project/Area Number: 18K15011
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 48020:Physiology-related
• Research Institution: Kyushu University
• Principal Investigator: Matsuzaki Fumiko 九州大学, 生体防御医学研究所, 助教 (10631773)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Keywords: 骨格筋 / インスリン / マルチオミクス

Outline of Final Research Achievements

In this project, skeletal muscle composition was analyzed in terms of molecular concentration/proportion, and their temporal variation over four hours after insulin administration. A molecular interaction network consisting of approximately 10,000 insulin-responsive molecules (nodes) was constructed based on this analysis. This network illustrates about 20,000 interactions (edges) among metabolites, RNAs, and proteins that changed in their phosphorylation or expression levels. Such a comprehensive analysis provided an overview of major regulatory processes within skeletal muscles in response to insulin. Our investigation model enabled the design of scalable methods that could integrate multiple sources to characterize and visualize biologically relevant molecular interactions.

Free Research Field

マルチオミクス

Academic Significance and Societal Importance of the Research Achievements

網羅的な分子定量から分子間相互作用ネットワークの構築・特徴解析・可視化までのワークフローの開発により、大規模データと既存知識を統合して生物組織において誘導される分子制御の全体像の理解が可能になった。このアプローチは拡張性が高いことから、適用範囲は骨格筋代謝に限らない。今後、より幅広い生命現象の制御メカニズム理解への応用が期待される。