2019 Fiscal Year Annual Research Report
Identification of immunomodulation molecules in Mycobacterial ESX secretory systems
| Project/Area Number |
18K15161
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| Research Institution | 公益財団法人結核予防会 結核研究所 |
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Principal Investigator |
郭 姿君 (GuoTzーChun) 公益財団法人結核予防会 結核研究所, 生体防御部 病理科, 研究員 (00813065)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Keywords | 結核菌 / PPE遺伝子 / 病原体宿主相互作用 / 宿主自然免疫 |
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| Outline of Annual Research Achievements |
Mycobacteria infection in host cells triggers a dynamic cascade of altered gene expression in both interacting organisms. To elucidate the functional role of ESX-5 secreted PE/PPE proteins in tuberculosis, two BCG deletion mutants, ppe26 or ppe27, were generated by mutagenesis. Macrophage cells were subjected to infection with wild-type and two mutated BCG variants. Dual RNA-seq was performed to unveil the altered gene expression both in the bacteria and infected host cells. Released cytokines were measured by multiplexed bead-based cytokine assay. The overall results from this study suggested the immunosuppressive role of PPE26 and PPE27. This confers the opportunity of manipulating PPE26/27 protein as potential candidate for drug intervention or vaccine development.
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