Establishment of human gastric organoid carcinogenesis model and target therapy focusing on ISX

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K15756
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Yokohama City University
• Principal Investigator: Sue Soichiro 横浜市立大学, 附属病院, 助教 (00738619)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: オルガノイド / 胃 / ヒト / ISX

Outline of Final Research Achievements

Human gastric organoids were cultured. Organoid based carcinogenesis model were tried, and ISX targeting therapy were screened by using the cultured organoids. Expression of E-cadherin and βcatenin on the cell membrane lesion of the organoid, and sheroid form changed to the form with unevenness. HGM, gastric marker, was positive at lumen of the organoid. The expression of ISX, gastric marker, and intestinal metaplasia marker of the organoids were checked by the mRNA analysis and fluorescent immunostaining. Passage of the organoids were possible, but long term passage were difficult. ISX expression were checked for screening ISX targeting candidate drugs with the organoids.

Free Research Field

消化器内科

Academic Significance and Societal Importance of the Research Achievements

本研究の目的はヒト胃オルガノイド発癌モデルを作成し、胃癌治療への応用につなげることである。本研究ではヒト胃オルガノイドを作成し短期間での継代培養に基づく実験が可能であることを明らかにした一方、期間内で長期培養の最適な条件をみつけることができず、ヒト胃オルガノイドの長期培養条件の検討が課題であることが明らかとなった。