The role of exosomes for the pathogenesis of COPD

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K15943
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53030:Respiratory medicine-related
• Research Institution: Tohoku University
• Principal Investigator: Makiguchi Tomonori 東北大学, 医学系研究科, 非常勤講師 (60815843)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: COPD

Outline of Final Research Achievements

We isolated the exosomes form lung fibroblasts of COPD patients and labelled the exosomes with PKH-67. We then performed the administration of the labelled exosomes onto cultured human lung bronchial epithelial cells. We found the incorporation of the labelled exosomes into the cells. We also performed the analysis of micro RNAs in the exosomes from the sera of COPD patients with or without the history of frequent exacerbation. We found that the microRNAs including hsa-miR-524-3p were significantly decreased in the COPD patients with frequent exacerbation, whereas the microRNAs including hsa-miR-100-5p、hsa-miR-1911-3p、hsa-miR-6764-5p were increased.

Free Research Field

呼吸器内科学

Academic Significance and Societal Importance of the Research Achievements

COPD患者血清エクソソームの解析では頻回増悪群にて減少している、microRNAとして、hsa-miR-524-3pなどの7つのmicroRNAが抽出された。一方、頻回増悪群にて上昇しているmicroRNAとして、hsa-miR-100-5p、hsa-miR-1911-3p、hsa-miR-6764-5pなどの31種のmicroRNAが低下していることが判明した。これらのエクソソームmicroRNAの解析によりCOPDの新規病態の解明につながり、かつ新規治療法の開発基礎につながる成果を得ることができた。