Analysis of thermoregulatory disorders using sweat gland organoids generated from human sweat gland stem cells

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K16028
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53050:Dermatology-related
• Research Institution: Osaka University
• Principal Investigator: Kurata Ryuichiro 大阪大学, 薬学研究科, 招へい教員 (50773115)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: 汗腺 / 幹細胞 / オルガノイド / 発汗 / 熱中症

Outline of Final Research Achievements

We screened the microenvironment that exerts the regenerative capacity of sweat gland stem cells using three-dimensional structural analysis of sweat glands. Profiling of the sweat gland basement membrane revealed that Laminin-332 and Laminin-511 were predominantly expressed in the duct and secretory portion of the sweat gland, respectively. In the secretory portion, integrin α6β1 which is a receptor of Laminin-511 was expressed, suggesting that Laminin-511 is implicated with the maintenance of sweat gland stem cells in the secretory portion of sweat glands. Furthermore, when the sweat gland stem cells were cultured under the shear stress by horizontal rotation, they acquired the proliferation activity.

Free Research Field

皮膚科学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は発汗障害解明の新しい糸口となり、将来の発汗改善薬の開発や熱中症患者の減少に貢献できる。