2018 Fiscal Year Research-status Report
Identification of novel biomarkers unique to the HIV-1 latent reservoir
| Project/Area Number |
18K16178
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| Research Institution | Kumamoto University |
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Principal Investigator |
MAHITI Macdonald 熊本大学, 国際先端医学研究機構, 客員講師 (50773321)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Keywords | HIV/AIDS |
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| Outline of Annual Research Achievements |
Although the antiretroviral therapy successfully prolongs lives of HIV-infected patients, the current therapy is insufficient to eradicate infected cells. Understanding the molecular characteristic and phenotype of the cells harboring latent provirus is thus crucial for achieving cure. However, distinguishing latently-infected cells from uninfected cells remains a challenge. Taking in consideration of the HIV pandemicity in my home country Tanzania, I first tried to establish a platform to access to blood specimens of HIV-infected patients who successfully treated by antiretroviral therapy at least for 2 years. I have successfully collected whole blood from such patients (about 100), separated PBMC, and send them to Japan after freezing. In parallel, I have developed the PCR-based assay to quantitate proviral DNA copy number to characterize the latent status of those patients. By screening >100 patients, I may be able to find better appropriate patients that might fit to further analyses in the next year.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The platform to access HIV-infected patients who match to the aims of this study has been successfully made.
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| Strategy for Future Research Activity |
I want to expand the number of patients for initial proviral load testing screening and then carefully choose certain numbers of patients that might be better appropriate for further analyses.
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| Causes of Carryover |
Because another funding only for travel was obtained and therefore I used that fund for travelling. Thereby I could use remaining expenses for lab experiments this year.
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[Journal Article] Resistance of major histocompatibility complex class B (MHC-B) to Nef-mediated downregulation relative to that of MHC-A is conserved among primate lentiviruses and influences antiviral T cell responses in HIV-infected individuals.2018
Author(s)
1.Mwimanzi F, Toyoda M, Mahiti M, Mann J, Martin J, Bangsberg D, Brockman M, Goulder P, Kirchhoff F, Brumme Z, Ndung’u T, Ueno T
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Journal Title
J Virol
Volume: 91
Pages: e00725-17
DOI
Peer Reviewed / Int'l Joint Research
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