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2020 Fiscal Year Annual Research Report

The human stomach microbiome and Helicobacter pylori infection.

Research Project

Project/Area Number 18K16182
Research InstitutionOita University

Principal Investigator

松本 昂  大分大学, 医学部, 助教 (50609667)

Project Period (FY) 2018-04-01 – 2021-03-31
Keywords胃マイクロバイオーム / ピロリ菌感染症 / 国際共同研究 / 次世代シーケンス
Outline of Annual Research Achievements

ヘリコバクター・ピロリ(ピロリ菌)は、人類の約半数の胃に感染する病原性細菌であり。慢性胃炎、胃癌、胃十二指腸潰瘍等の疾患発症に関わる。しかし、実際は、ピロリ菌感染者の数%しか胃癌を発症せず、また、生涯を通じて殆ど症状が現れない不顕性感染例が存在するなど、疾患の発症機序には不明な点が多い。そこで我々は、胃独自に生息する細菌叢に注目し、次世代シーケンスによる16Sメタゲノム解析により、胃マイクロバイオームを明らかにしピロリ菌感染症との関連を明らかにすることを研究目的とした。さらに、これらゲノム解析手法を応用し、ピロリ菌の病原性等について詳細な検討した。
本研究では、細菌存在数の非常に少ない胃粘膜組織からのDNA抽出方法を確立し、次世代シーケンサーによる16Sメタゲノム解析を実施、Qiime等の解析パイプラインにてOperational Taxonomic Unit(OTU)を決定し、胃マイクロバイオームとピロリ菌感染症、胃癌さらに地域性との関連について解析を行った。ピロリ菌OUT数、病理評価および抗ピロリ菌血清抗体値等を比較した結果、ピロリ菌OTU数は、胃粘膜の炎症や萎縮と相関性が高く、また、ピロリ菌非感染患者では、地域ごとに特徴的な細菌叢を構成していることが伺えた。さらに、胃癌症例と非胃癌症例を比較した検討では、胃癌発症率が世界で最も高いモンゴルにおいて、予想に反して、胃癌患者におけるピロリ菌OTU量の割合が低く、ピロリ菌以外の特定の細菌の増加が確認されるなど、非常に興味深い結果が得られた。即ち、これら細菌が胃癌発症に何らかの影響を与えている可能性が考えられ、PICRUStにより機能予測解析を実施し、学術論文を報告した。また、モンゴルやインドネシアを含む計8か国との連携した国際共同研究ネットワークを築き、ワークショップを開催するなど、研究体制の拡充や教育にも貢献できた。

  • Research Products

    (21 results)

All 2021 2020 Other

All Int'l Joint Research (6 results) Journal Article (10 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 10 results,  Open Access: 6 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] MNUMS(モンゴル)

    • Country Name
      MONGOLIA
    • Counterpart Institution
      MNUMS
  • [Int'l Joint Research] Universitas Airlangga/University of Muhammadiyah Surabaya/University of Indonesia(インドネシア)

    • Country Name
      INDONESIA
    • Counterpart Institution
      Universitas Airlangga/University of Muhammadiyah Surabaya/University of Indonesia
  • [Int'l Joint Research] Baylor College of Medicine(米国)

    • Country Name
      U.S.A.
    • Counterpart Institution
      Baylor College of Medicine
  • [Int'l Joint Research] University of Mbujimayi/University of Kinshasa/General Referential Hospital of Bukavu(コンゴ民主共和国)

    • Country Name
      DEM. REP. OF CONGO
    • Counterpart Institution
      University of Mbujimayi/University of Kinshasa/General Referential Hospital of Bukavu
    • # of Other Institutions
      2
  • [Int'l Joint Research] Technical University of Kenya/Kenyatta University/Aga Khan Hospital University(ケニア)

    • Country Name
      KENYA
    • Counterpart Institution
      Technical University of Kenya/Kenyatta University/Aga Khan Hospital University
    • # of Other Institutions
      2
  • [Int'l Joint Research]

    • # of Other Countries
      8
  • [Journal Article] Advantage of 16S rRNA amplicon sequencing in Helicobacter pylori diagnosis2021

    • Author(s)
      Gantuya Boldbaatar、El Serag Hashem B.、Saruuljavkhlan Batsaikhan、Azzaya Dashdorj、Matsumoto Takashi、Uchida Tomohisa、Oyuntsetseg Khasag、Oyunbileg Nyamdorj、Davaadorj Duger、Yamaoka Yoshio
    • Journal Title

      Helicobacter

      Volume: e12790 Pages: -

    • DOI

      10.1111/hel.12790

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers2021

    • Author(s)
      Phuc Bui Hoang、Tuan Vo Phuoc、Dung Ho Dang Quy、Binh Tran Thanh、Tung Pham Huu、Tri Tran Dinh、Thuan Ngo Phuong Minh、Van Khien Vu、Trang Tran Thi Huyen、Akada Junko、Matsumoto Takeshi、Yamaoka Yoshio
    • Journal Title

      Scientific Reports

      Volume: 11 Pages: 4584

    • DOI

      10.1038/s41598-021-83862-1

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Discovery of unique African Helicobacter pylori CagA-multimerization motif in the Dominican Republic2020

    • Author(s)
      Ono Takaaki、Cruz Modesto、Nagashima Hiroyuki、Subsomwong Phawinee、Akada Junko、Matsumoto Takashi、Uchida Tomohisa、Suzuki Rumiko、Hosking Celso、Abreu Jos? A Jim?nez、Yamaoka Yoshio
    • Journal Title

      World Journal of Gastroenterology

      Volume: 26 Pages: 7118~7130

    • DOI

      10.3748/wjg.v26.i45.7118

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Whole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma2020

    • Author(s)
      Mwangi Catherine、Njoroge Stephen、Tshibangu-Kabamba Evariste、Moloo Zahir、Rajula Allan、Devani Smita、Matsumoto Takashi、Nyerere Kimang’a、Kariuki Samuel、Revathi Gunturu、Yamaoka Yoshio
    • Journal Title

      Toxins

      Volume: 12 Pages: 556~556

    • DOI

      10.3390/toxins12090556

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Biofilm Formation and Antibiotic Resistance Phenotype of Helicobacter pylori Clinical Isolates2020

    • Author(s)
      Fauzia Kartika Afrida、Miftahussurur Muhammad、Syam Ari Fahrial、Waskito Langgeng Agung、Doohan Dalla、Rezkitha Yudith Annisa Ayu、Matsumoto Takashi、Tuan Vo Phuoc、Akada Junko、Yonezawa Hideo、Kamiya Shigeru、Yamaoka Yoshio
    • Journal Title

      Toxins

      Volume: 12 Pages: 473~473

    • DOI

      10.3390/toxins12080473

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gut-Homing CD4+ T Cells Are Associated with the Activity of Gastritis in HIV-Infected Adults2020

    • Author(s)
      Sato Hidenori、Ota Yasunori、Kido Yasutoshi、Matsumoto Takashi、Matsubara Yasuo、Matano Tetsuro、Hirata Yoshihiro、Kawana-Tachikawa Ai、Yamaoka Yoshio、Yotsuyanagi Hiroshi、Adachi Eisuke
    • Journal Title

      AIDS Research and Human Retroviruses

      Volume: 36 Pages: 910~917

    • DOI

      10.1089/AID.2020.0086

    • Peer Reviewed
  • [Journal Article] High Antibiotic Resistance of Helicobacter pylori and Its Associated Novel Gene Mutations among the Mongolian Population2020

    • Author(s)
      Azzaya Dashdorj、Gantuya Boldbaatar、Oyuntsetseg Khasag、Davaadorj Duger、Matsumoto Takashi、Akada Junko、Yamaoka Yoshio
    • Journal Title

      Microorganisms

      Volume: 8 Pages: 1062~1062

    • DOI

      10.3390/microorganisms8071062

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Next-Generation Sequencing of the Whole Bacterial Genome for Tracking Molecular Insight into the Broad-Spectrum Antimicrobial Resistance of Helicobacter pylori Clinical Isolates from the Democratic Republic of Congo2020

    • Author(s)
      Tshibangu-Kabamba Evariste、Ngoma-Kisoko Patrick de Jesus、Tuan Vo Phuoc、Matsumoto Takashi、Akada Junko、Kido Yasutoshi、Tshimpi-Wola Antoine、Tshiamala-Kashala Pascal、Ahuka-Mundeke Steve、Mumba Ngoy Dieudonn、Disashi-Tumba Ghislain、Yamaoka Yoshio
    • Journal Title

      Microorganisms

      Volume: 8 Pages: 887~887

    • DOI

      10.3390/microorganisms8060887

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gastric microbiota and Helicobacter pylori in Indonesian population2020

    • Author(s)
      Miftahussurur Muhammad、Waskito Langgeng Agung、El‐Serag Hashem B、Ajami Nadim J.、Nusi Iswan Abbas、Syam Ari Fahrial、Matsumoto Takashi、Rezkitha Yudith Annisa Ayu、Doohan Dalla、Fauzia Kartika Afrida、Maimunah Ummi、Sugihartono Titong、Uchida Tomohisa、Yamaoka Yoshio
    • Journal Title

      Helicobacter

      Volume: 25 Pages: e12695

    • DOI

      10.1111/hel.12695

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Gastric mucosal microbiota in a Mongolian population with gastric cancer and precursor conditions2020

    • Author(s)
      Gantuya Boldbaatar、El Serag Hashem B.、Matsumoto Takashi、Ajami Nadim J.、Uchida Tomohisa、Oyuntsetseg Khasag、Bolor Dashdorj、Yamaoka Yoshio
    • Journal Title

      Alimentary Pharmacology & Therapeutics

      Volume: 51 Pages: 770~780

    • DOI

      10.1111/apt.15675

    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Gastric Microbiota: From Sampling to Obtain the Sequence2021

    • Author(s)
      Takashi Matsumoto
    • Organizer
      International workshop Gastroenterology Research Opportunities in Antimicrobial Resistance, Metagenomic, Clinical Surveillance and Wet-Lab Experiment at ITD, UNAIR
    • Invited
  • [Presentation] Helicobacter pylori infection and gastric microbiota2020

    • Author(s)
      Takashi Matsumoto, Yoshio Yamaoka
    • Organizer
      Aga Khan university Symposium on H. pylori infection
    • Invited
  • [Presentation] The human gastric microbiome and Helicobacter pylori infection2020

    • Author(s)
      Takashi Matsumoto
    • Organizer
      Mongolian Digestive Disease Week 2020
    • Int'l Joint Research
  • [Presentation] Helicobacter pylori infection and gastric microbiota2020

    • Author(s)
      Takashi Matsumoto
    • Organizer
      KEMRI Annual Scientific and Health Conference
    • Int'l Joint Research
  • [Remarks] 大分大学医学部環境・予防医学講座

    • URL

      http://phealth2.wp.med.oita-u.ac.jp/

URL: 

Published: 2021-12-27  

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