Effect of incretin GIP on bone metabolism in vivo

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K16197
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 54040:Metabolism and endocrinology-related
• Research Institution: Kyoto University
• Principal Investigator: Shimazu-Kuwahara Satoko 京都大学, 医学研究科, 特定助教 (80805771)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: インクレチン / 骨

Outline of Final Research Achievements

We analyzed the phenotype of osteoblast-specific GIP receptor-deficient mice. In osteoblast-specific GIP receptor-deficient mice, there was no significant difference in the expression of GIP receptors in the following organs: bone, islets, brain, intestine, visceral fat, subcutaneous fat, and brown fat. Whole body bone length was measured using soft X-rays, but no significant difference was observed. No significant differences were found in glucose tolerance, GIP, or insulin secretion capacity. μCT analysis was performed to analyze bone density, but no significant differences were found. We confirmed GIP receptor expression in osteoblast culture, osteoclast culture, and cell line, but GIP receptor expression could not be confirmed using primary culture.

Free Research Field

インクレチン

Academic Significance and Societal Importance of the Research Achievements

骨組織におけるGIP（Gastric inhibitory polypeptide）の生体内での役割を明らかにするために、骨芽細胞特異的GIP受容体欠損マウスを作製した。骨芽細胞のGIP作用についてin vivoで検討した結果、GIPから骨組織へのシグナルは、直接骨に発現する受容体を介するのではなく、間接的に作用している可能性があり、介在するその他の液性因子などが考えられた。