Development of a circulating miRNA-based diagnostic signature forearly detection of gastric cancer

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K16261
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55010:General surgery and pediatric surgery-related
• Research Institution: Kumamoto University
• Principal Investigator: Yamamura Kensuke 熊本大学, 病院, 特任助教 (10816507)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: 胃癌 / マイクロRNA / signature

Outline of Final Research Achievements

Currently endoscopic surveillance is the gold standard for gastric cancer (GC) diagnosis, but remains as an ineffective screening strategy due to its invasiveness and associated costs. Herein, by integrating high-throughput data analysis, followed by comprehensive tissue and serum validations, we aimed to establish a circulating miRNA-based diagnostic signature for early detection of GC. We have developed a robust diagnostic marker for GC through integration of comprehensive high-throughput data analysis as well as clinical validation involving over 1200 GC samples across 6 independent cohorts. Collectively, our data demonstrates clinical significance of circulating-miRNA-signature for early detection of GC.

Free Research Field

消化器外科学

Academic Significance and Societal Importance of the Research Achievements

現在胃癌の診断はコストが高く侵襲を伴っている。安価で非侵襲的なバイオマーカーの探索は以前からなされているが、確立されたものは少なくいわば古くて新しい課題である。本研究では、オンラインで入手可能な複数の胃癌組織の網羅的解析データファイル及びグローバルに集められた600例以上の臨床サンプルを用いて、より精度が高く侵襲の少ない胃癌診断のためのマイクロRNA signatureを開発した。本研究で得られた結果は、末梢血から早期胃癌を診断可能なバイオマーカーの開発につながる可能性がある。