Establishment of PDX model in pancreatic neuroendocrine tumor

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K16345
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: OGAWA KOSUKE 東京医科歯科大学, 医学部附属病院, 助教 (40618112)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 膵癌 / 膵神経内分泌腫瘍 / PDXモデル

Outline of Final Research Achievements

We created a PDX model in 4 patients with pancreatic cancer, 6 with pancreatic neuroendocrine tumors, and 1 with pancreatic SPN. In 3 of 4 cases transplanted for pancreatic cancer, we created a PDX model of the 3rd generation or later that can be used in the experiment and succeeded in establishing it. Furthermore, among 3 cases of pancreatic cancer PDX model, lung metastasis was confirmed in 2 cases, and a system was created in which spontaneous lung metastasis can be seen. This is currently being submitted as a treatise. In addition, if two rare pancreatic neuroendocrine tumors can reach the second generation (the stage where the tumor grows to 1 cm and is transplanted to the next NSG mouse), and then transplanted to create the third generation, it will actually be possible. Can be used for animal experiments.

Free Research Field

肝胆膵外科

Academic Significance and Societal Importance of the Research Achievements

膵癌のＰＤＸモデル3系統の樹立に成功し、自然に肺転移を起こす膵癌のPDXモデル２系統の作成に成功した。また、希少がんである膵神経内分泌腫瘍2系統でのＰＤＸモデル2系統の樹立中であり、人からマウスへの腫瘍移植の次段階である、マウスからマウスへの移植まで成功している。ＰＤＸモデルは患者腫瘍の微小環境を忠実に再現していて、動物実験の中でも臨床研究の前段階の前臨床モデルとして重要視されている。これを膵癌と膵神経内分泌腫瘍で樹立したことの社会的意義は大きい。