Is it possible to diagnose intestinal ischemia using Raman imaging?

2022 Fiscal Year Final Research Report

• Project/Area Number: 18K16515
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Shimane University
• Principal Investigator: Shimojo Yoshihide 島根大学, 学術研究院医学・看護学系, 助教 (70725069)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Keywords: 腸管虚血

Outline of Final Research Achievements

Using a rat model of intestinal ischemia, we wanted to analyze the differences in Raman spectra between ischemic and normal areas of the mucosa that could not be seen with the naked eye, but we were unable to identify specific proteins (proteins induced by hypoxia). In the future, we will analyze from the plasma membrane surface because lipid characteristics are mixed in. In addition, it is necessary to analyze Raman spectroscopy focusing not on the intestinal tract itself but on the hematopoietic components generated by intestinal necrosis.

Free Research Field

Acute Care Surgery

Academic Significance and Societal Importance of the Research Achievements

ベッドサイドにおける腸管虚血・壊死の診断法としてラマン分光法の診断精度を検証するべく研究を進めたが、目的を果たすことには至らなかった。本研究の成果が得られた際には検査移動がしづらい鎮静、鎮痛下かつ集中治療管理中の傷病者に発生した腸管虚血・壊死の診断能力が向上し患者様へのメリットがあるものと予想された。