The role of MCT in the regeneration mechanism of skeletal muscle

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K16650
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: University of Yamanashi
• Principal Investigator: TAKAYAMA Yoshihiro 山梨大学, 大学院総合研究部, 臨床助教 (20596994)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: 骨格筋 / MCT / PAR2 / 肥満細胞 / 脂肪分化

Outline of Final Research Achievements

It is reported the gathering of mast cells has been reported in the early stage of the muscle regeneration process. In this experiment, the proliferation of mouse myoblast cell (C2C12) was increased in a concentration-dependent manner by stimulation with mast cell-derived tryptase (MCT). In addition, a group in which MCT was administered using a system for muscle differentiation under horse serum was obtained with the result that muscle differentiation was promoted as compared with the control. Next, myoblasts (G8) were used to observe fat differentiation. Unlike muscle differentiation, MCT administration suppressed adipose differentiation. As the results, MCT promoted proliferation and muscle differentiation in myoblasts and suppressed adipose differentiation. These results suggest that MCT may have a protective/promoting effect on muscle cells.

Free Research Field

骨格筋の再生メカニズム

Academic Significance and Societal Importance of the Research Achievements

近年、腰痛患者や腰椎変性疾患では体幹筋力に、萎縮と脂肪変性が認められることが多く報告され 、腰痛メカニズムにおいて体幹筋の脂肪変性が注目されている。筋肉の再生・変性メカニズムにおいては筋衛星細胞（骨格筋幹細胞；筋芽細胞）が大きな役割を持つことが予想され、今回の実験を行った。その結果、肥満細胞由来トリプターゼ（ＭＣＴ）が筋細胞に対して保護的/促進的に働いている可能性を示唆された。ターゲットとなる分子の創薬を行って新規治療を開発し人類の健康寿命の長期化に寄与できるものと考える。