2020 Fiscal Year Research-status Report
Discovery of genes essential for male fertility using CRISPR/Cas9-based genome editing technology
| Project/Area Number |
18K16735
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| Research Institution | Osaka University |
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Principal Investigator |
Lu Yonggang 大阪大学, 微生物病研究所, 特任助教(常勤) (00817033)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Keywords | male reproduction / male contraception / sperm / sperm-egg fusion / CRISPR/Cas9 |
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| Outline of Annual Research Achievements |
In 2020, I have successfully published multiple articles regarding the study of sperm-egg fusion and made important progress towards the understanding of the molecular functions of other proteins required for male reproduction. The discovery of new molecules required for sperm-egg fusion (including SPACA6, TMEM95, SOF1 and FIMP) is of great significance for unraveling the detailed mechanisms underpinning this important reproductive events. Due to its spatiotemporal dynamic nature, the study of the gamete fusion process is extremely challenging. By identifying more molecules required for the membrane fusion between sperm and eggs, we will be able to acquire a comprehensive understanding of this process.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
I successfully published the study on sperm-egg fusion and identified more molecules involved in the fusion process. I will be able to acquire more profound insights into the cellular and molecular mechanism underpinning this process.
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| Strategy for Future Research Activity |
I would like to carry out more in-depth analyses on the molecules involved in sperm-egg fusion, so as to acquire a deeper understanding of the mechanism underlying this important reproductive event. Through CRISPR/Cas9-based knockout strategy, my ultimate goal is to identify the bona fide fusogen that mediates the membrane fusion between sperm and eggs.
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Research Products
(6 results)
