2021 Fiscal Year Annual Research Report
Discovery of genes essential for male fertility using CRISPR/Cas9-based genome editing technology
| Project/Area Number |
18K16735
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| Research Institution | Osaka University |
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Principal Investigator |
Lu Yonggang 大阪大学, 免疫学フロンティア研究センター, 特任助教(常勤) (00817033)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Keywords | Male reproduction / CRISPR/Cas9 / Sperm-egg fusion / Fertilization / Knockout / Mouse model / Male contraception / Spermatology |
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| Outline of Annual Research Achievements |
During the four-year funding period, the applicant has generated multiple knockout mouse lines lacking testis-specific genes and analyzed the resultant phenotype. This study demonstrates the feasibility and efficiency of using the CRISPR/Cas9-mediated knockout strategy to identify genes essential for male reproduction. In addition to the biological relevance, the identified molecules may serve as biomarkers for male infertility and potential targets for non-hormonal male contraception. Through the large-scale in vivo screening, we have discovered essential genes Lrrc23, C2cd6, Sof1, Tmem95, and Fimp, which play roles in regulating sperm motility and sperm-egg fusion. The relevant research outcomes have been published in international peer-reviewed journals.
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Research Products
(5 results)
