Regeneration of peri-implant tissue by multicellular spheroid-containing cementoblast cell sheet

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K17133
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Fukuoka Dental College
• Principal Investigator: SATO AYAKO 福岡歯科大学, 口腔歯学部, 医員 (70803998)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: インプラント / 再生 / 細胞シート

Outline of Final Research Achievements

Dental implant therapy must prompt to recover the harmonious structure in the periodontal tissue, through a direct functional connection between ordered, living bone and the surface of a load-carrying implant. In culture experiment, cell sheet-based regenerative techniques, using human periodontal ligament stem cells (HPLSC), were applied to facilitate a cementum-coating on the implant body and a favorable regeneration of periodontal ligament cells which can connect with surface of both regenerative cementum and alveolar bones. Furthermore, I examined that DNA/protamine-based scaffolds enhanced regeneration of vertical alveolar bone defects of the dog mandible. From these findings, I suggest that the HPLSC sheets may be one of the most promised techniques for harmonious regeneration of the periodontal complex including the implant body.

Free Research Field

補綴

Academic Significance and Societal Importance of the Research Achievements

本研究は細胞シートの応用によるセメント芽細胞とインプラント体とのインテグレーション誘導およびDNA/Protamineスキャフォールドを利用した骨再生の誘導により、新たなインプラント周囲組織の再生方法を検討した。セメント芽細胞を基盤とした歯周組織様の構造を再現することでインプラント周囲に生体と調和のとれた周囲組織再生の可能性が示された。また、セメント芽細胞シートを応用することで歯周組織の再生など、他分野への応用も可能と考えられる。