Activation of endogenous jaw bone cells by deposition-type apatite

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K17153
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Kagoshima University
• Principal Investigator: Tanaka Kenko 鹿児島大学, 医歯学総合研究科, 客員研究員 (00610049)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: 間葉系幹細胞 / アパタイト / ヒアルロン酸 / 骨補填剤

Outline of Final Research Achievements

The purpose of this study is to develop a new biomaterial that can be used as a scaffold for mesenchymal stem cells (MSCs). As a result of evaluating the biocompatibility of ACH and MSCs in vitro, cell proliferation and spreading of MSCs were confirmed on ACH. Next, with the aim of developing a new biomaterial in which various ions were deposited on carbonate apatite, we tried to deposit divalent metal ions on carbonate apatite disks. We have succeeded in producing strontium (Sr) coated carbonate apatite. Next, MSCs were seeded on the Sr-coated carbonate apatite. As a result, it was confirmed that the MSCs adhered on the Sr-coated carbonate apatite. It was revealed that the adhered MSCs showed the same morphology as the cells cultured on the uncoated carbonate apatite.

Free Research Field

再生医学

Academic Significance and Societal Importance of the Research Achievements

アパタイトコートヒアルロン酸(ACH)、およびストロンチウム（Sr）コート炭酸アパタイトが抗原性なく、内在性顎骨細胞としてのMSCを賦活化するための足場となることで骨化促進作用が計られ、さらに骨形成能を持つMSCと合わせることで、骨増生法の最大の欠点である治療期間の長期化を解決することができる。