2022 Fiscal Year Annual Research Report
Differentiation and physiological function analysis of osteoclasts using Raman spectroscopy and its application to elucidation of the pathogenesis of osteonecrosis of the jaw
| Project/Area Number |
18K17175
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
Marin Elia 京都工芸繊維大学, 材料化学系, 准教授 (10814014)
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Keywords | ラマン / 骨 / 顎骨壊死 |
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| Outline of Annual Research Achievements |
By using Raman spectroscopy, we were able to understand the changes in structure and microstructure of bone samples due to the effects of osteonecrosis, and in particular the loss of mineralization and the changes in the composition of the bone collagen. These information were obtained by samples acquired from patients, and we were able to notice differences in osteonecrosis caused by alcohol and osteonecrosis caused by the assumption of steroids, observing that alcohol causes also healthy bone tissue to reduce its cristallinity and average "quality". Overall, Raman spectroscopy proved to be a powerful tool in the study of human bone, able to assess the quality of both the mineral and the organic fraction. Moreover, Raman spectroscopy could be used as an imaging tool, giving accurate chemical maps of the structure and composition of large areas of bone, even when severely affected by osteonecrosis. Due to the large volume of data, the results were studied by using statistical tools such as principal component analysis in order to be able to correctly estimate the statistical relevance of each effect. Lastly, the large volume of data could be used to produce of database of osteonecrotic bone tissue and healthy bone tissue to be used as reference for future research and for diagnostic applications.
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