Elucidating the anti-colitic mechanisms of Rice Bran supplementation via nutriomics

2018 Fiscal Year Research-status Report

• Project/Area Number: 18K17976
• Research Institution: Keio University
• Principal Investigator: Aw WanPing 慶應義塾大学, 政策・メディア研究科(藤沢), 特任助教 (20736441)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: rice bran / colitis / metabolome / gut microbiome

Outline of Annual Research Achievements

Mice were treated eith normal diet or 5%, 10%, 20% RB supplemented diet, and administered DSS from days 0 to 10. In Control group, body weight was decreased as colitis developed. This was suppressed by RB intake. We also observed that the abundance of Clostridiaceae was higher in RB-fed mice.
Next,normal diet-fed mice feces or RB-fed mice feces were transplanted into germ-free mice, and normal diets was given, and the same DSS experiment was performed. Fecal microbiota transplantation from RB-fed mice suppressed colitis. This strongly suggests that RB suppresses colitis through gut microbiota modulation.
The fecal metabolome profile was dynamically changed by RB intake. Enrichment analysis revealed that RB intervention enriched the tryptophan metabolism pathway.

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

We have successfully identified the metabolites involved in colitis suppression.

Strategy for Future Research Activity

In the RB-related metabolite treatment experiment, we have observed colitis suppression. We will next perform animal experiment in the metabolite receptor-knockout mice.

Causes of Carryover

As our animal experiment went on smoothly as expected, we would use the incurring amount to be used in the next fiscal year.