2019 Fiscal Year Annual Research Report
Elucidating the anti-colitic mechanisms of Rice Bran supplementation via nutriomics
| Project/Area Number |
18K17976
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| Research Institution | Keio University |
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Principal Investigator |
Aw WanPing 慶應義塾大学, 政策・メディア研究科(藤沢), 特任助教 (20736441)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Keywords | Rice Bran / DSS colitis / Metabolome / Microbiome |
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| Outline of Annual Research Achievements |
In order to verify the dose dependent effects of Rice Bran (RB) supplementation, mice were fed a 5% RB, 10% RB, 20% RB-supplemented AIN-93G basal diet and tap water for 1 week, then 2.0% DSS-containing water for 10 days respectively. During the experimental period, fecal samples from all the mice were collected daily for 16S rRNA gene analysis and CE-TOFMS-based metabolome profiling. 10 days after DSS administration, RB-fed mice suppressed colitis dose-dependently. The abundance of Clostridium spp. was significantly higher in RB-fed mice. Fecal metabolome analysis showed that the amount of the tryptophan metabolite was significantly increased in RB-fed mice as compared to control mice. DSS-induced colitis was suppressed in germ-free mice transplanted with fecal microbiota from RB-fed mice.
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