Development of a high-throughout drug recovery assay of stress for natural compounds screening

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K17993
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 59040:Nutrition science and health science-related
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Saiki Papawee 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (10803934)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: stress / IL-6 / IL-10 / bioluminescence

Outline of Final Research Achievements

In this study, I studied microbiota-brain-gut axis via immune system in chronic mild stress. I found that immune system and gut microbiota were changed in PAWW stress mice. Finally, I selected IL6 and IL10 to be bio-marker of PAWW stress mice. I developed new and stable RAW 264.7 derived dual-color IL-6/gapdh and IL-10/gapdh reporters. This assay allowed us to easily determine relative IL-6 and IL-10 levels with 96-well plate within one step. I evaluated the relative IL-6 and IL-10 levels in the LPS-induced stable cells testing 52 natural products by real-time bioluminescence monitoring. By this screening assay, I found that 1`S-1`-acetoxychavicol from greater galangal could reduce IL-6 level and increase IL-10 level. Finally, I can suggest that these stable cells by real-time monitoring could serve as a screening assay for anti-inflammatory activity and may be used to discover new drugs against stress.

Free Research Field

immune system

Academic Significance and Societal Importance of the Research Achievements

In the future, we may reduce animal experiments by this assay for screening compounds or drugs. However, this finding is meaningful not only for academics, but it may lead the people concern that stress could make immune system change, and cause the disease, and herb to reboot immune system.