Investigation of biomolecular activity in liposomes mimicking the surface of cell membranes

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K19157
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 37:Biomolecular chemistry and related fields
• Research Institution: Gunma University
• Principal Investigator: Kamiya Koki 群馬大学, 大学院理工学府, 助教 (70612315)
• Project Period (FY): 2018-06-29 – 2020-03-31
• Keywords: リポソーム / リン脂質二重膜 / リン脂質非対称膜 / 膜タンパク質 / マイクロドメイン / マイクロデバイス

Outline of Final Research Achievements

Giant lipid vesicles with asymmetric lipid bilayer which mimics a plasma membrane of eukaryotic cells were generated by applying a jet flow against a planar asymmetric lipid bilayer (pulsed jet flow method). The plasma membranes are also composed of microdomains and glycolipids. In this study, to improve emulations of the plasma membrane of eukaryotic cells, cell-sized asymmetric lipid vesicles with microdomains and glycolipids were generated by the pulsed jet flow method.

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

膜ドメインや糖鎖を導入した細胞膜を模倣したリン脂質非対称膜上で、ペプチドや膜タンパク質等の生体分子の相互作用を観察することで、これらの生体分子の素反応を理解できる。よって、精密な生体分子の素反応の理解は、細胞システムのメカニズムの解明や薬剤の活性部位の特定に役立つ。