2019 Fiscal Year Final Research Report
Elucidation of methane- and butanol-production pathways by novel white-rot fungi
| Project/Area Number |
18K19229
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2020-03-31
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| Keywords | 白色腐朽菌 / メタン / ブタノール / バイオリファイナリー |
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| Outline of Final Research Achievements |
We screened each mutant which produced methane or butanol, from hyper lignin degrading fungus Phanerochaete sordida YK-624. Although no butanol-producing mutant was detected, methane-producing mutant was isolated. In order to analyze the mutation site of the genome DNA, whole genome sequence was carried out by MiSeq. We could not determine the mutation site since the cell of P. sordida YK-624 was coenocyte and the mutation was occurred in only a certain nucleus.
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| Free Research Field |
環境生化学
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| Academic Significance and Societal Importance of the Research Achievements |
元々メタン産生能を有さない白色腐朽担子菌においても、突然変異処理を施すことにより、メタン産生能を示すことが明らかとなった。これは、木質バイオマスから本菌を用いることでメタンが産生可能となることを示しており、メタン生合成経路を解明し、関連各種遺伝子を高発現させることで、ワンポットで木材よりメタンを産生可能な菌の育種が可能となる。この様な菌の作出は、低炭素社会の実現の一翼を担うものである。
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