• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2020 Fiscal Year Final Research Report

Development of Fundamental Technique for 4D Chemical Nucleomics

Research Project

  • PDF
Project/Area Number 18K19402
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 47:Pharmaceutical sciences and related fields
Research InstitutionOsaka University

Principal Investigator

Hori Yuichiro  大阪大学, 工学研究科, 准教授 (00444563)

Project Period (FY) 2018-06-29 – 2021-03-31
KeywordsChemical Nucleomics / dCas9 / TALE / PYP / Hybrid probe
Outline of Final Research Achievements

In this study, we developed a technique for fluorescent detection of target DNA sequences with the aim of developing a fundamental tool for 4D nucleome analysis to visualize genome dynamics. For this purpose, we will construct a hybrid probe consisting of a DNA-binding protein used for genome editing and a synthetic nucleic acid-binding dye using our protein labeling technology. This hybrid probe was shown to have the ability to bind to the target telomeric DNA sequence and increase the fluorescence intensity.

Free Research Field

ケミカルバイオロジー、蛍光イメージング

Academic Significance and Societal Importance of the Research Achievements

蛍光タンパク質を用いたDNA配列の検出技術は、バックグラウンドシグナルの大きなものであるが、本研究のハイブリッドプローブは、DNAへの結合に伴い蛍光強度が増大するため、より高精度に検出できる。DNAの高精度な検出をもとにした4Dヌクレオソーム解析は、ゲノム動態を精密に明らかにすることでき、遺伝子発現制御や疾病発症のメカニズムの解明に繋がる。この観点から、本研究では、ゲノム動態解明のための基盤ツールの開発に成功しており、生命科学や医学の発展に貢献するといえる。

URL: 

Published: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi