• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2019 Fiscal Year Final Research Report

Investigation of inner mitochondrial collapsing in response to acute cardiac overstretch.

Research Project

  • PDF
Project/Area Number 18K19435
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 48:Biomedical structure and function and related fields
Research InstitutionJikei University School of Medicine

Principal Investigator

Kusakari Yoichiro  東京慈恵会医科大学, 医学部, 准教授 (80338889)

Co-Investigator(Kenkyū-buntansha) 三尾 寧  東京慈恵会医科大学, 医学部, 教授 (00266686)
立花 利公  東京慈恵会医科大学, 医学部, 教授 (80163476)
岩本 武夫  東京慈恵会医科大学, 医学部, 教授 (90568891)
Project Period (FY) 2018-06-29 – 2020-03-31
Keywordsミトコンドリア / 心筋 / 張力 / 過伸展
Outline of Final Research Achievements

We performed acute overstretch on cardiac papillary muscle to investigate the mechanisms in morphological changes in mitochondria. We overstretched right ventricle papillary muscle within 2 seconds. We analyzed the morphological changes using a Transmission electron microscopy (TEM). In TEM study, inner mitochondrial empty space was found after overstretch, indicating that inner mitochondria cristae were susceptible to deterioration. We investigated the effect of 1) overstretch release, 2) changes in Ca concentrations and 3) inhibiting mPTP (mitochondrial permeability transition pore) on acute overstretch. Releasing overstretch and changes in Ca concentration did not alter the inner mitochondrial collapsing. The inner mitochondrial collapsing was 20% reduced by inhibiting mPTP. It indicates that the mitochondrial collapsing was partly due to by Ca entering to mitochondrial inner space through mPTP.

Free Research Field

心筋の病態生理学

Academic Significance and Societal Importance of the Research Achievements

これまで経験的に知られていた心筋過伸展の分子メカニズムの一部を解明した。今後は病態時心筋の過伸展による影響を検討する。このことは、心不全の発症メカニズムを探る貴重なデータになると確信している。過伸展による微細形態構造変化への影響は未知な部分が多いため、今後の研究で心不全治療や抑制を来す、新たなターゲットとなる分子機構や創薬に繋がると思われる。心移植になる前の予防にも重要であると考える。

URL: 

Published: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi