In vitro mouse small cell lung cancer model using isolated lung epithelium

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K19480
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 50:Oncology and related fields
• Research Institution: Kumamoto University
• Principal Investigator: ITO Takaaki 熊本大学, 大学院生命科学研究部(医), 教授 (70168392)
• Project Period (FY): 2018-06-29 – 2021-03-31
• Keywords: 小細胞肺癌 / マウスモデル / オルガノイド

Outline of Final Research Achievements

We have established mouse small cell lung cancer (SCLC) model combinding Tp53 KO, Rb1KO and inacitivation of Notch signaling (Hes1 KO) mice, and isolated epithelia obtained from the mouse SCLC model, and cultured them within Matrigel. After about 100 days of cultivation, epithellial proliferative lesions ocurred. After 200days of cultivation, neoplastic cells grew out of the Matrigel with adhesiove or floating pattern. These tumor cells were inoculated in the subcutaneous tissue of the immune-deficinet mice, and histological features of the xenotransplantd tumors were examined. Various histological types of lung cancer including squamous cell carcinoma, adenocarcinoma and small cell carcinoma were detected.

Free Research Field

病理学

Academic Significance and Societal Importance of the Research Achievements

私達は、Tp53KO, Rb1KO,Notchシグナル不活化による新たな小細胞肺癌マウスモデルを作成し、さらに、このマウスの胎仔肺上皮から、in vitroでの小細胞肺癌発生モデルの開発にも成功した。このことは、この難治性の腫瘍の発生過程を試験管内で再現でき、この腫瘍の発生過程の研究、また、予防、治療、などへと発展できる研究モデルを開発したことになり、学術的にも社会的にも有意義な成果といえる。