2022 Fiscal Year Final Research Report

Development of a novel analytical method for intracranial injuries and diseases Using NMR modal analysis of postmortem cerebrospinal fluid.

Research Project

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Project/Area Number	18K19705
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 58:Society medicine, nursing, and related fields
Research Institution	Nippon Medical School
Principal Investigator	Hirakawa Keiko 日本医科大学, 医学部, 非常勤講師 (30165162)
Co-Investigator(Kenkyū-buntansha)	金涌佳雅日本医科大学, 大学院医学研究科, 大学院教授 (80465343) 小池薫京都大学, 医学研究科, 名誉教授 (10267164) 足立好司日本医科大学, 医学部, 准教授 (00231928) 山崎峰雄日本医科大学, 医学部, 教授 (10277577) 早川秀幸日本医科大学, 大学院医学研究科, 研究生 (10373052) 柚木知之京都大学, 医学研究科, 准教授 (50639094)
Project Period (FY)	2018-06-29 – 2023-03-31
Keywords	死体 / 髄液 / NMR / モード解析
Outline of Final Research Achievements	Postmortem cerebrospinal fluid (CSF), which can be collected during postmortem examination, is a useful sample for forensic diagnosis to determine the presence or absence of subarachnoid hemorrhage by whether it is bloody or clear. Since postmortem CSF is often routinely collected in forensic practice, it is expected to be utilized developmentally for diagnosis. In this research, postmortem CSF from postmortem examinations and forensic autopsies, in which the cause of death is known, was used to perform NMR modal analysis to verify its association with the cause of death. It was difficult to make a detailed association of cause of death in each case based solely on the results of NMR modal analysis of the postmortem CSF obtained in this study. However, NMR modal analysis of the postmortem CSF suggested the possibility of identifying sudden death, death due to inflammatory disease, and death from external causes as the cause of death, i.e., the pathological condition prior to death.
Free Research Field	法医学
Academic Significance and Societal Importance of the Research Achievements	異状死の死因究明は、法医学における重要な使命であるが、法医解剖や死後画像診断は必ずしも十分に普及しているとは言い難いのが現状である。死後髄液の採取自体は容易で特別な装置を要するものではないので、死後髄液のNMRモード特性から、頭蓋内病変の有無、死因との関連性の情報を抽出できれば、法医実務への貢献は大きいと考える。解剖検査による死因究明を凌駕する精度は困難ではあろうが、死体検案での信頼度の高い死因究明の方法として、社会的意義は高いだろう。また本研究を通じた、死後髄液の死因の病態生理学的分析は、法医病理学の基礎研究の新たな対象試料として髄液が期待できる可能性を示唆させる学術的意義を有すると認める。