• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2019 Fiscal Year Final Research Report

Imaging analysis of exercise-governed intramuscular microenvironments required for immunometabolic regulation

Research Project

  • PDF
Project/Area Number 18K19722
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
Research InstitutionTohoku University

Principal Investigator

Kanzaki Makoto  東北大学, 医工学研究科, 准教授 (10272262)

Project Period (FY) 2018-06-29 – 2020-03-31
Keywords運動 / 骨格筋 / 代謝 / 免疫 / イメージング / マイオカイン / GLUT4 / 好中球
Outline of Final Research Achievements

By establishing a novel intravital imaging system, we successfully revealed the importance of intramuscular paracrine/autocrine systems involving contracting myofibers, adjacent vascular endothelial cells and recruited neutrophils, which are triggered by muscle contractile activity and reliant upon CX3CL1/fractalkine-mediated signals. Specifically, the intravital imaging using transgenic mouse harboring skeletal muscle-specific GLUT4-EGFP expression and Quantum-dot-labelled neutrophils demonstrated that the fractalkine-mediated signals are required for both neutrophil accumulation and enhanced GLUT4 translocation in the working skeletal muscles. It is thus concluded that fractalkine is a key factor for organizing a neutrophil‐mediated favorable immunometabolic niche by orchestrating intramuscular vascular and immune responses in working skeletal muscles.

Free Research Field

運動生理学

Academic Significance and Societal Importance of the Research Achievements

本研究によって新たに開発した「運動筋ニッチにおける免疫代謝微小環境の生体イメージング解析システム」は、活動中の骨格筋内局所において惹起される異種細胞間の機能連携とその生理的重要性を理解する上で極めて有用な優れた解析系である。広範な骨格筋研究へと応用が可能であり、筋研究領域に多大な貢献をもたらすと期待される。また、本研究により運動筋ニッチがつかさどる運動免疫ネットワーク制御機構の一端を明らかにできた。今後、2型糖尿病などの生活習慣病、ロコモ、加齢時における「運動筋ニッチ」の状態を精査していくことで、病態生理的観点からも「運動筋ニッチ」の重要性を明らかになれば、新規の治療標的となる可能性が高い。

URL: 

Published: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi