2023 Fiscal Year Final Research Report
Elucidation of physiological role of four CNS barriers in human based on the establishment of next generation transporter assay method
| Project/Area Number |
18KK0446
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research (A))
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Hiroshima University (2023)
Tohoku University (2018-2022) |
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Principal Investigator |
Uchida Yasuo 広島大学, 医系科学研究科(薬), 教授 (70583590)
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| Project Period (FY) |
2019 – 2023
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| Keywords | トランスポーター / 探索法 / 局在解析法 / 絶対定量法 / 血液脳関門 / 血液クモ膜関門 / 高深度定量プロテオミクス |
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| Outline of Final Research Achievements |
In this study, in collaboration with the Institute for Molecular Systems Biology at the Swiss Federal Institute of Technology, which possesses state-of-the-art omics technology, we established a new methodology for transporter analysis including the molecular identification of new transporters and comprehensive determination of transporter localization. This has led to the identification of new transporter molecules in the central nervous system (CNS) barriers and the elucidation of the different roles of the four CNS barriers. Due to the Copid19 pandemic, the planned number of travel days was not met, but we were able to introduce technology and generate results by making extensive use of web conferencing with overseas collaborators, and finally, the initially planned research purpose was achieved.
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| Free Research Field |
分子システム薬剤学
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| Academic Significance and Societal Importance of the Research Achievements |
トランスポーターの創薬研究は、他の分子群（GPCRなど）よりも遅れている。原因として、①分子同定の方法がlow throughputであること、②トランスポーター様の構造のタンパク質を見いだせても輸送機能を効率よく解析する方法が不十分であること、③細胞膜局在（輸送方向）を解析する方法がlow throughputであること、④物質輸送への寄与を定量的に評価する方法がlow throughputであることが挙げられる。本研究は、これらの課題を解決する普遍的なトランスポーター解析戦略を構築した点で意義がある。
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