2011 Fiscal Year Final Research Report
Molecular mechanisms underlying nuclear reprogramming of somatic cells
| Project/Area Number |
19002014
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| Research Category |
Grant-in-Aid for Specially Promoted Research
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AOI Takashi 京都大学, iPS細胞研究所, 教授 (00546997)
NAKAGAWA Masato 京都大学, iPS細胞研究所, 講師 (10379539)
TAKAHASHI Kazutoshi 京都大学, iPS細胞研究所, 講師 (80432326)
OKITA Keisuke 京都大学, iPS細胞研究所, 講師 (90512434)
YOSHIDA Yoshinori 京都大学, iPS細胞研究所, 特定拠点講師 (20447965)
WATANABE Akira 京都大学, iPS細胞研究所, 特定拠点助教 (60506765)
YAMAMOTO Takuya 京都大学, iPS細胞研究所, 特定拠点助教 (60546993)
KNUT Woltjen 京都大学, iPS細胞研究所, 特定拠点助教 (50589489)
KOYANAGI Michiyo 京都大学, iPS細胞研究所, 特定研究員 (90432327)
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| Project Period (FY) |
2007 – 2011
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| Keywords | 再生医学 / 幹細胞 / 分化多能性 / 核初期化 / 転写因子 |
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| Research Abstract |
iPS cells can be generated by introduction of four reprogramming factors into somatic cells. We have found that chimera mice produced with c-Myc show high tumorigenicity. To overcome this issue, we could generate iPS cells without Myc under the modified conditions. But Myc minus iPS cells showed less pluripotency compared to iPS cells generated with c-Myc. Next, we explored the factors which could replace the c-Myc function. We found L-Myc as a candidate. We could confirm that L-Myc enhances the efficiency of iPS generation and that the tumorigenicity of L-Myc is hardly observed. So, we can obtain the safer iPS cells using L-Myc. We established the system to examine the safety of iPS cells using the iPS cells-derived neuronal cells. We also established the differentiation methods of iPS cells into hepatocytes, blood cells, and cardiomyocytes in vitro. Next generation sequencer(NGS) is very powerful tool for analysis of genetic properties of iPS cells. We have analyzed the gene expression, DNA methylation, changes of splicing pattern in iPS cells using NGS
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