2011 Fiscal Year Final Research Report
Molecular Design of Oxygenases Applicable to Synthetic Chemistry
| Project/Area Number |
19105004
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAGIMA Hiroshi 名古屋大学, 大学院・理学研究科, 准教授 (00283151)
NAGANO Shingo 鳥取大学, 大学院・工学研究科, 教授 (60286440)
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| Project Period (FY) |
2007 – 2011
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| Keywords | 生物無機化学 / ヘム蛋白質 |
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| Research Abstract |
The study has achieved creation of novel artificial oxygenases founded on a heme protein deriving from a thermophile or a cytochrome P450 protein that utilizes H2O2 as the oxidant. A newly developed method,"Decoy molecule" succeeded to rationally modify the active site of the P450 protein in addition to conventional mutagenesis techniques. The artificial oxygenases exerted high catalytic activity and/or thermal tolerance which were not achieved by ever reported artificial oxygenases. Results in the study provide ideas and methodology to design artificial oxygenases aiming practical uses and might be applicable to artificial enzymes of other activities.
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[Journal Article] Incorporation of Phebox Rhodium Complex into apo-Myoglobin Affords Stable Organometallic Protein Showing Unprecedented Arrangement of the Complex in the Cavity2007
Author(s)
Y. Satake, S. Abe, S. Okazaki, N. Ban, T. Hikage, T. Ueno, H. Nakajima, A. Suzuki, T. Yamane, H. Nishiyama, Y. Watanabe
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Journal Title
Organometallics.
Volume: 26
Pages: 4904-4908
DOI
Peer Reviewed
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