Comparative genomics of the conditionally dispensable chromosomes controlling plant infection in Alternaria alternata pathogens

2010 Fiscal Year Final Research Report

• Project/Area Number: 19108001
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Nagoya University
• Principal Investigator: TSUGE Takashi 名古屋大学, 生命農学研究科, 教授 (30192644)
• Co-Investigator(Kenkyū-buntansha): KODAMA Motoichirou 鳥取大学, 農学部, 教授 (00183343)
• Project Period (FY): 2007 – 2010
• Keywords: 植物病原糸状菌 / 宿主特異的毒素 / 病原性 / CD染色体 / 寄生性進化

Research Abstract

The CD(Conditionally Dispensable) chromosomes of plant pathogenic fungi are essential for the disease-causing capacity on host plants, but not for growth on media. We performed the comparative genomics analysis of CD chromosomes essential for host-specific pathogenicity of three pathogens of Alternaria alternata. The CD chromosomes were found to consist of large syntenic regions among the three chromosomes and pathogen-specific regions determining host-specific pathogenicity. This result suggests that the syntenic regions are the core of the original, dispensable chromosome, and that pathogenicity genes were integrated into the original chromosome.

Research Products

• [Journal Article] Functional analysis of the ceramide synthase gene ALT7, a homolog of the disease resistance gene Asc1, in the plant pathogen Alternaria alternata (2012)
  • Author(s): Kheder, A. A., Akagi, Y., Tsuge, T., and Kodama, M
  • Journal Title: J. Plant Pathol. Microbiol. Pages: S2-001
  • DOI: DOI:10.4172/2157-7471.S2-001.
  • Peer Reviewed
• [Journal Article] Contribution of peroxisomes to secondary metabolism and pathogenicity in the fungal plant pathogen Alternaria alternata (2010)
  • Author(s): Imazaki, A., Tanaka, A., Harimoto, Y., Yamamoto, M., Akimitsu, K., Park, P., and Tsuge, T
  • Journal Title: Eukaryot. Cell Volume: 9 Pages: 682-694
  • DOI: DOI:10.1128/EC.00369-09
  • Peer Reviewed
• [Journal Article] ACTTS3 encoding a polyketide synthase is essential for the biosynthesis of ACT-toxin and pathogenicity in the tangerine pathotype of Alternaria alternata (2010)
  • Author(s): Miyamoto, Y., Masunaka, A., Tsuge, T., Yamamoto, M., Ohtani, K., Fukumoto, T., Gomi, K., Peever, T. L., Tada, Y., Ichimura, K., and Akimitsu, K
  • Journal Title: Mol. Plant-Microbe Interact. Volume: 23 Pages: 406-414
  • DOI: DOI:10.1094/MPMI-23-4-0406
  • Peer Reviewed
• [Journal Article] Role of the host-selective ACT-toxin synthesis gene ACTTS2 encoding an enoyl-reductase in pathogenicity of the tangerine pathotype of Alternaria alternata. (2010)
  • Author(s): Ajiro, N., Miyamoto, Y., Masunaka, A., Tsuge, T., Yamamoto, M., Ohtani, K., Fukumoto, T., Gomi, K., Peever, T. L., Izumi, Y., Tada, Y., and Akimitsu, K.
  • Journal Title: Phytopathology Volume: 100 Pages: 120-126
  • DOI: DOI:10.1094/PHYTO-100-2-0120
  • Peer Reviewed
• [Journal Article] Chromosome constitution of hybrid strains constructed by protoplast fusion between the tomato and strawberry pathotypes of Alternaria alternata (2009)
  • Author(s): Akagi, Y., Taga, M., Yamamoto, M., Tsuge, T., Fukumasa-Nakai, Y., Otani, H., and Kodama, M
  • Journal Title: J. Gen. Plant Pathol. Volume: 75 Pages: 101-109
  • DOI: DOI:10.1007/s10327-009-0149-1
  • Peer Reviewed
• [Journal Article] Horizontal chromosome transfer : a mechanism for the evolution and differentiation of a plant pathogenic fungus (2009)
  • Author(s): Akagi, Y., Akamatsu, H., Otani, H., and Kodama, M
  • Journal Title: Eukariot. Cell Volume: 8 Pages: 1732-1738
  • DOI: DOI:10.1128/EC.00135-09
  • Peer Reviewed
• [Journal Article] Function of genes encoding acyl-CoA synthetase and enoyl-CoA hydratase for host-selective ACT-toxin biosynthesis in the tangerine pathotype of Alternaria alternata (2009)
  • Author(s): Miyamoto, Y., Isshiki, Y., Honda, A., Masunaka, A., Tsuge, T., Yamamoto, M., Ohtani, K., Fukumoto, T., Gomi, K., Peever, T. L., and Akimitsu, K
  • Journal Title: Phytopathology Volume: 99 Pages: 369-377
  • DOI: DOI:10.1094/PHYTO-99-4-0369
  • Peer Reviewed
• [Journal Article] Functional analysis of a multi-copy host-selective ACT-toxin biosynthesis gene in the tangerine pathotype of Alternaria alternata using RNA silencing (2008)
  • Author(s): Miyamoto, Y., Masunaka, A., Tsuge, T., Yamamoto, M., Ohtani, K., Fukumoto, T., Gomi, K., Peever, T. L., and Akimitsu, K
  • Journal Title: Mol. Plant-Microbe Interact. Volume: 21 Pages: 1591-1599
  • DOI: DOI:10.1094/MPMI-21-12-1591
  • Peer Reviewed
• [Journal Article] Multiple copies of AMT2 are prerequisite for the apple pathotype of Alternaria alternata to produce enough AM-toxin for expressing pathogenicity (2008)
  • Author(s): Harimoto, Y., Tanaka, T., Kodama, M., Yamamoto, M., Otani, H., and Tsuge, T.
  • Journal Title: J. Gen. Plant Pathol. Volume: 74 Pages: 222-229
  • DOI: DOI:10.1007/s10327-008-0089-1
  • Peer Reviewed
• [Journal Article] Expression profiles of genes encoded by the supernumerary chromosome controlling AM-toxin biosynthesis and pathogenicity in the apple pathotype of Alternaria alternata (2007)
  • Author(s): Harimoto, Y., Hatta, R., Kodama, M., Otani, H., and Tsuge, T
  • Journal Title: Mol. Plant-Microbe Interact. Volume: 20 Pages: 1463-1476
  • DOI: DOI:10.1094/MPMI-20-12-1463
  • Peer Reviewed
• [Presentation] 病原性Alternaria alternataにおけるglobal regulator LaeAホモログの機能と病理学的役割 (2012)
  • Author(s): 高尾和実,赤木靖典,柘植尚志,播本佳明,尾谷浩,児玉基一朗
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-03-30
• [Presentation] 植物病原糸状菌における二次代謝産物生合成と病原性-ゲノム解析からみた多様性と進化- (2010)
  • Author(s): 児玉基一朗
  • Organizer: 日本農薬学会第8回農薬バイオサイエンス研究会「農薬科学の未来を考える-植物・微生物研究の最前線」
  • Place of Presentation: 京都
  • Year and Date: 2010-12-03
• [Presentation] Pathogenicity chromosomes in toxin-dependent necrotrophic plant pathogens (2010)
  • Author(s): Kodama, M., Akagi, Y., Harimoto, Y., Otani, H., Yamamoto, M., Taga, M. and Tsuge, T
  • Organizer: The 9th International Mycological Congress
  • Place of Presentation: Edinburgh, Scotland, UK
  • Year and Date: 2010-08-06
• [Presentation] A Zn(II) 2Cys6-type transcription regulator embedded in the AM-toxin biosynthetic gene cluster negatively controls the toxin biosynthesis in the apple pathotype of Alternaria alternata (2009)
  • Author(s): Harimoto, Y., Kodama, M., Yamamoto, M., Otani, H., and Tsuge, T.
  • Organizer: The 25th Fungal Genetics Conference, Pacific Grove
  • Place of Presentation: CA, USA
  • Year and Date: 20090317-22
• [Presentation] Evolution of pathogenic strategies in a toxin-dependent necrotrophic pathogen Alternaria alternata (2009)
  • Author(s): Kodama, M., Akagi, Y., Egusa, M., Otani, H., Tsuge, T., Yamamoto, M. and Taga, M.
  • Organizer: The 1st Japan-Korea Joint Symposium
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2009-10-30
• [Presentation] 植物寄生菌の病原性を決定するCD染色体 (2009)
  • Author(s): 柘植尚志,張裕介,間瀬千晶,新城明久,八田理恵子,伊藤芳,田中孝欣,播本佳明,赤木靖典,児玉基一朗,山本幹博,秋光和也,尾谷浩
  • Organizer: 日本菌学会シンポジウム「植物寄生菌類における病原性の獲得と多様性」
  • Place of Presentation: 鳥取
  • Year and Date: 2009-08-20
• [Presentation] Alternaria alternata病原菌の植物寄生性を決定するCD染色体 (2009)
  • Author(s): 柘植尚志,張裕介,間瀬千晶,新城明久,八田理恵子,伊藤芳,田中孝欣,播本佳明,赤木靖典,小松龍太,佐藤昭之,児玉基一朗,山本幹博,秋光和也,尾谷浩
  • Organizer: 日本植物病理学会大会シンポジウム「植物免疫のゲノミクスとポストゲノミクス」
  • Place of Presentation: 山形
  • Year and Date: 2009-03-27
• [Presentation] メロンつる割病菌とイチゴ黒斑病菌の病原性を制御する相同な転写制御因子の同定 (2008)
  • Author(s): 倉田智彬,倉橋真,播本佳明,張裕介,飯田祐一郎,柘植尚志
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 松江
  • Year and Date: 2008-04-27
• [Presentation] A gene cluster on a conditionally dispensable chromosome controlling AAL-toxin biosynthesis and pathogenicity in the tomato pathotype of Alternaria alternata (2007)
  • Author(s): Kodama, M., Akagi, Y., Akamatsu, H., Otani, H., Yamamoto, M. and Tsuge, T
  • Organizer: The 13th International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Sorrento, Italy
  • Year and Date: 20070722-26
• [Book] 第6章病原体の病原性発現機構3.糸状菌の病原性遺伝子の発現機構.植物病理学(眞山志,難波成任編) (2009)
  • Author(s): 柘植尚志
  • Total Pages: 183-186
  • Publisher: 文永堂出版
• [Book] A gene cluster on a conditionally dispensable chromosome controlling AAL-toxin synthesis and pathogenicity in the tomato pathotype of Alternaria alternata. In : Biology of Plant-Microbe Interactions Vol.6(M. Lorito, et al., eds.) (2008)
  • Author(s): Kodama, M., Akagi, Y., Akamatsu, H., Otani, H., Yamamoto, M. and Tsuge, T
  • Total Pages: 18
  • Publisher: International Society of Molecular Plant-Microbe Interactions, St. Paul, MN, USA