Molecular basis of Rac GTPase in plant innate immunity

2011 Fiscal Year Final Research Report

• Project/Area Number: 19108005
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: SHIMAMOTO Ko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (10263427)
• Co-Investigator(Kenkyū-buntansha): KAWASAKI Tsutomu 奈良先端科学技術大学院大学, バイオサエンス研究科, 准教授 (90283936) ; WONG Hann ling 奈良先端科学技術大学院大学, バイオサエンス研究科, 助教 (00403353) ; TERADA Rie 名城大学, 農学部, 准教授 (30137799) ; KAWANO Yoji 奈良先端科学技術大学院大学, バイオサエンス研究科, 助教 (00406175)
• Project Period (FY): 2007 – 2011
• Keywords: 植物免疫

Research Abstract

We have previously reported that small GTPase OsRac1 plays important roles in rice innate immunity. However, it is largely unknown how OsRac1 induces immune responses. In this study, we showed that OsRac1 forms a complex termed Defensome with various downstream proteins, including R proteins, pattern recognition receptors, a scaffolding protein, and cochaperone proteins. OsRac1 also plays a dual role in the induction of ROS production through direct interactions with NADPH oxidases and the suppression of the ROS scavenger. Moreover, we demonstrate here that Defensome acts as an important regulator in rice innate immunity.

Research Products

• [Journal Article] The bHLH Rac Immunity1(RAI1) Is Activated by OsRac1 via OsMAPK3 and OsMAPK6 in Rice Immunity (2012)
  • Author(s): Kim SH, Oikawa T, Kyozuka J, Wong HL, Umemura K, Kishi-Kaboshi M, Takahashi A, Kawano Y, Kawasaki T, Shimamoto K.
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 740-54
  • Peer Reviewed
• [Journal Article] SWAP70 functions as a Rac/Rop guanine nucleotide-exchange factor in rice (2012)
  • Author(s): Yamaguchi K, Imai K, Akamatsu A, Mihashi M, Hayashi N, Shimamoto K, Kawasaki T
  • Journal Title: Plant J Volume: 70(3) Pages: 389-97
  • Peer Reviewed
• [Journal Article] Reactive oxygen species production and activation mechanism of the rice NADPH oxidase OsRbohB (2012)
  • Author(s): akahashi S, Kimura S, Kaya H, Iizuka A, Wong HL, Shimamoto K, Kuchitsu K
  • Journal Title: J Biochem
  • Peer Reviewed
• [Journal Article] Emerging roles of molecular chaperones in plant innate immunity (2011)
  • Author(s): Chen L and Shimamoto K
  • Journal Title: J. Gen. Plant Pathol Volume: 77(1) Pages: 1-9
  • Peer Reviewed
• [Journal Article] Activation of a Rac GTPase by the NLR family disease resistance protein Pit plays a critical role in rice innate immunity (2010)
  • Author(s): Kawano, Y., Akamatsu, A., Hayashi, K., Housen, Y., Okuda, J., Yao, A., Nakashima, A., Takahashi, H., Yoshida, H., Wong, H. L., Kawasaki, T. and Shimamoto, K.
  • Journal Title: Cell Host Microbe Volume: 7 Pages: 362-375
  • Peer Reviewed
• [Journal Article] The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity (2010)
  • Author(s): Chen, L., Hamada, S., Fujiwara, M., Zhu, T., Thao, N. P., Wong, H. L., Krishna, P., Ueda, T., Kaku, H., Shibuya, N., Kawasaki, T. and Shimamoto, K.
  • Journal Title: Cell Host Microbe Volume: 7 Pages: 185-196
  • Peer Reviewed
• [Journal Article] Analysis of the Rac/Rop small GTPase family in rice : expression (2010)
  • Author(s): Chen, L., Shiotani, K., Togashi, T., Miki, D., Aoyama, M., Wong, H. L., Kawasaki, T. and Shimamoto, K.
  • Journal Title: subcellular localization and role in disease resistance Volume: 51 Pages: 585-595
  • Peer Reviewed
• [Journal Article] Sekiguchi lesion gene encodes a cytochrome P450 monooxygenase that catalyzes conversion of tryptamine to serotonin in rice (2010)
  • Author(s): Fujiwara, T., Maisonneuve, S., Isshiki, M., Mizutani, M., Chen, L., Wong, H. L., Kawasaki, T. and Shimamoto, K.
  • Journal Title: J Biol Chem Volume: 285 Pages: 11308-11313
  • Peer Reviewed
• [Journal Article] Structure of the N-terminal regulatory domain of a plant NADPH oxidase and its functional implications (2010)
  • Author(s): Oda, T., Hashimoto, H., Kuwabara, N., Akashi, S., Hayashi, K., Kojima, C., Wong, H. L., Kawasaki, T., Shimamoto, K., Sato, M. and Shimizu, T.
  • Journal Title: J Biol Chem Volume: 285 Pages: 1435-1445
  • Peer Reviewed
• [Journal Article] Sending ROS on a bullet train (2009)
  • Author(s): Wong, HL. and Shimamoto, K.
  • Journal Title: Sci. Signal Volume: 29 Pages: 60
  • Peer Reviewed
• [Journal Article] Proteome analysis of detergent-resistant membranes(DRMs) associated with OsRac1-mediated innate immunity in rice (2009)
  • Author(s): Fujiwara, M., Hamada, S., Hiratsuka, M., Fukao, Y., Kawasaki, T. and Shimamoto, K.
  • Journal Title: Plant Cell Physiol Volume: 50 Pages: 1191-1200
  • Peer Reviewed
• [Journal Article] Crystallographic characterization of the N-terminal domain of a plant NADPH oxidase (2008)
  • Author(s): Oda, T., Hashimoto, H., Kuwabara, N., Hayashi, K., Kojima, C., Kawasaki, T., Shimamoto, K., Sato, M. and Shimizu, T.
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun Volume: 64 Pages: 867-869
  • Peer Reviewed
• [Journal Article] RACK1 functions in rice innate immunity by interacting with the Rac1 immune complex (2008)
  • Author(s): Nakashima, A., Chen, L., Thao, N. P., Fujiwara, M., Wong, H. L., Kuwano, M., Umemura, K., Shirasu, K., Kawasaki, T. and Shimamoto, K.
  • Journal Title: Plant Cell Volume: 20 Pages: 2265-2279
  • Peer Reviewed
• [Journal Article] RAR1 and HSP90 form a complex with Rac/Rop GTPase and function in innate-immune responses in rice (2007)
  • Author(s): Thao, N. P., Chen, L., Nakashima, A., Hara, S., Umemura, K., Takahashi, A., Shirasu, K., Kawasaki, T. and Shimamoto, K.
  • Journal Title: Plant Cell Volume: 19 Pages: 4035-4045
  • Peer Reviewed
• [Journal Article] Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension (2007)
  • Author(s): Wong, H. L., Pinontoan, R., Hayashi, K., Tabata, R., Yaeno, T., Hasegawa, K., Kojima, C., Yoshioka, H., Iba, K., Kawasaki, T. and Shimamoto, K.
  • Journal Title: Plant Cell Volume: 19 Pages: 4022-4034
  • Peer Reviewed
• [Journal Article] Rice Pti1a negatively regulates RAR1-dependent defense responses (2007)
  • Author(s): Takahashi, A., Agrawal, G. K., Yamazaki, M., Onosato, K., Miyao, A., Kawasaki, T., Shimamoto, K. and Hirochika, H.
  • Journal Title: Plant Cell Volume: 19 Pages: 2940-2951
  • Peer Reviewed
• [Journal Article] Calcium-dependent protein kinases regulate the production of reactive oxygen species by potato NADPH oxidase (2007)
  • Author(s): Kobayashi, M., Ohura, I., Kawakita, K., Yokota, N., Fujiwara, M., Shimamoto, K., Doke, N. and Yoshioka, H.
  • Journal Title: Plant Cell Volume: 9 Pages: 1065-1080
  • Peer Reviewed
• [Presentation] Defensome model for rice innate immunity (2011)
  • Author(s): Ko Shimamoto
  • Organizer: 2nd International Conference on Biotic Plant Interactions
  • Place of Presentation: 中国
  • Year and Date: 2011-11-12
• [Presentation] Defensome model for rice innate immunity (2011)
  • Author(s): Ko Shimamoto
  • Organizer: International Union of Microbiological Societies
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-06
• [Presentation] Molecular signaling in rice immunity (2010)
  • Author(s): Ko Shimamoto
  • Organizer: 6th International Rice Genetics Symposium
  • Place of Presentation: フィリピン
  • Year and Date: 2010-11-19
• [Presentation] OsRac1-mediated innate immunity in rice (2009)
  • Author(s): Ko Shimamoto
  • Organizer: 6th Rice Functional Genomics
  • Place of Presentation: 韓国
  • Year and Date: 2009-11-10
• [Presentation] OsRac1 is involved in both PTI and ETI in rice (2009)
  • Author(s): Ko Shimamoto
  • Place of Presentation: カナダ
  • Year and Date: 2009-07-24
• [Presentation] Rice innate immunity (2008)
  • Author(s): Ko Shimamoto
  • Organizer: Bio Hanoi2007
  • Place of Presentation: ベトナム
  • Year and Date: 2008-12-18
• [Presentation] Rice innate immunity (2008)
  • Author(s): Ko Shimamoto
  • Organizer: 13th International Congress of Plant Pathology
  • Place of Presentation: イタリア
  • Year and Date: 2008-08-24
• [Presentation] Signaling in OsRac1-mediated rice immunity (2008)
  • Author(s): Ko Shimamoto
  • Organizer: 3th International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: イタリア
  • Year and Date: 2008-07-21
• [Book] 蛋白質核酸酵素増刊 (2010)
  • Author(s): 河野、島本
  • Total Pages: 95-100
  • Publisher: 共立出版
• [Book] 化学と生物 (2009)
  • Author(s): 藤原、島本
  • Total Pages: 378-380
  • Publisher: 日本農芸化学会
• [Book] 実験医学増刊 (2009)
  • Author(s): Wongら
  • Total Pages: 80-83
  • Publisher: 羊土者
• [Remarks]
  • URL: http://bsw3.aist-nara.ac.jp/simamoto/simamoto.html