2009 Fiscal Year Final Research Report
Development of diagnostic tests for infectious diseases of mice and rats by microsphere fluorescent immunoassay
| Project/Area Number |
19200033
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YAGAMI Ken-ichi University of Tsukuba, 大学院・人間総合科学研究科, 教授 (40166476)
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| Co-Investigator(Kenkyū-buntansha) |
KUNITA Satoshi 筑波大学, 大学院・人間総合科学研究科, 講師 (10195472)
ARIKAWA Jiro 北海道大学, 大学院・医学研究科, 教授 (10142704)
OHSAWA Kazutaka 長崎大学, 学内共同利用施設, 教授 (90244756)
GOTO Kazuo 帝京大学, 医療技術学部, 准教授 (00205593)
IKE Fumio (独)理化学研究所, バイオリソースセンター, 研究員 (40183157)
TAKAKURA Akira (財)実験動物中央研究所, ICLASモニタリングセンター, 部長 (60167484)
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| Research Collaborator |
KATO Kanako 筑波大学, 生命科学動物資源センター, 研究員
KAMEDA Syuko (財)実験動物中央研究所, 研究員
ISHIDA Tomoko (財)実験動物中央研究所, 研究員
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| Project Period (FY) |
2007 – 2009
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| Keywords | 実験動物 / 微生物検査 / 血清診断 / マイクロビーズ法 / マウス / ラット |
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| Research Abstract |
Microsphere fluorescent immunoassay (MFI) allows simultaneous detection of antibodies to multiple rodent pathogens using a small volume of sera. In the present study, we examined the specificity and sensitivity of MFI in serologic diagnosis for infections on mice and rats. Antibodies to major 9 pathogens in mice and rats were measured by MFI using recombinant and inactivated antigens. MFI succeeded in multiplex detection of antibodies to the above-mentioned pathogens in a single reaction well without decreasing its specificity and sensitivity. MFI is highly suitable for serodiagnosis in laboratory mice and rats.
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[Journal Article] Specific and quantitative detection of PCR products from Clostridium piliforme, Helicobacter bilis, H. hepaticus, and mouse hepatitis virus infected mouse samples using a newly developed electrochemical DNA chip2007
Author(s)
Goto K, Horiuchi H, Shinohara H, Motegi K, Hashimoto K, Hongo S, Gemma N, Hayashimoto N, Itoh T, Takakura A.
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Journal Title
J Microbiol Methods 69(1)
Pages: 93-9
Peer Reviewed
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[Journal Article] Lymphocytic choriomeningitis infection undetected by dirty-bedding sentinel monitoring and revealed after embryo transfer of an inbred strain derived from wild mice2007
Author(s)
Ike F, Bourgade F, Ohsawa K, Sato H, Morikawa S, Saijo M, Kurane I, Takimoto K, Yamada YK, Jaubert J, Berard M, Nakata H, Hiraiwa N, Mekada K, Takakura A, Itoh T, Obata Y, Yoshiki A, Montagutelli X.
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Journal Title
Comp Med. 57(3)
Pages: 272-81
Peer Reviewed
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