2009 Fiscal Year Final Research Report
Mutagenesis using transposon system in C57BL/6 mice
| Project/Area Number |
19200034
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University |
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Principal Investigator |
TAKEDA Junji Osaka University, 先端科学イノベーションセンター, 教授 (50163407)
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| Co-Investigator(Kenkyū-buntansha) |
HORIE Kyoji 大阪大学, 医学系研究科, 准教 (30333446)
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| Project Period (FY) |
2007 – 2009
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| Keywords | トランスポゾンシステム / ミュタゲネーシス / C57BL / 6マウス / 表現型解析 / 病態モデル |
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| Research Abstract |
We have succeeded generation of mutant mice with Sleeping Beauty transposon system. In this project, we established such system in C57BL/6 mice. To stably express SB transposase, we targeted the transposase gene into Rosa26 locus using C57BL/6 ES cells. We are able to distribute the ES cell line.
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[Journal Article] SMOC1 is essential for ocular and limb development in humans and mice.2011
Author(s)
Okada I, Hamanoue H, Terada K, Tohma T, Megarbane A, Chouery E, Abou-Ghoch J, Jalkh N, Cogulu O, Ozkinay F, Horie K, Takeda J, Furuichi T, Ikegawa S, Nishiyama K, Miyatake S, Nishimura A, Mizuguchi T, Niikawa N, Hirahara F, Kaname T, Yoshiura K, Tsurusaki Y, Doi H, Miyake N, Furukawa T, Matsumoto N, Saitsu H.
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Journal Title
Am J Hum Genet. 88
Pages: 30-41.
Peer Reviewed
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[Journal Article] Diacylglycerol kinase β knockout mice exhibit lithium-sensitive behavioral abnormalities.2010
Author(s)
Kakefuda K, Oyagi A, Ishisaka M, Tsuruma K, Shimazawa M, Yokota K, Shirai Y, Horie K, Saito N, Takeda J, Hara H.
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Journal Title
PLoS One. 5
Pages: e13447.
Peer Reviewed
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[Journal Article] Essential role of neuron-enriched diacylglycerol kinase (DGK), DGKbeta in neurite spine formation, contributing to cognitive function.2010
Author(s)
Shirai Y, Kouzuki T, Kakefuda K, Moriguchi S, Oyagi A, Horie K, Morita SY, Shimazawa M, Fukunaga K, Takeda J, Saito N, Hara H.
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Journal Title
Peer Reviewed
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[Journal Article] Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro.2010
Author(s)
Yagita K, Horie K, Koinuma S, Nakamura W, Yamanaka I, Urasaki A, Shigeyoshi Y, Kawakami K, Shimada S, Takeda J, Uchiyama Y.
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Journal Title
Proc Natl Acad Sci USA. 107(8)
Pages: 3846-3851.
Peer Reviewed
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[Journal Article] A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in the mouse genome.2009
Author(s)
Kokubu C, Horie K, Abe K, Ikeda R, Mizuno S, Uno Y, Ogiwara S, Ohtsuka M, Isotani A, Okabe M, Imai K, Takeda J
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Journal Title
Nature Genet. 41
Pages: 946-952.
Peer Reviewed
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