Cellular effects of precisely controlled spatio-temporal electrical stimulation

2009 Fiscal Year Self-evaluation Report

• Project/Area Number: 19200036
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: The University of Tokyo
• Principal Investigator: JIMBO Yasuhiko The University of Tokyo, 大学院・新領域創成科学研究科, 教授 (20372401)
• Project Period (FY): 2007 – 2010
• Keywords: 脳・神経 / 神経科学 / 細胞・組織 / 分子認識 / ナノバイオ

Research Abstract

神経・心筋細胞等電気的にアクティブな組織を対象にin vitro系で個々の細胞を可視化した細胞集団を構成し、単一細胞のレベルで時空間的に精密に制御した電気刺激を印加してその効果につき調べる。このような時空間精密電気刺激の効果は短期的な細胞興奮現象の誘導にとどまらず、長期的に遺伝子発現過程に関与することもあり得ると考えている。以上の状況認識に基づき、本研究では、時空間精密電気刺激の効果を(1)短期的な細胞群興奮制御(心筋細胞系)、(2)中期的な神経回路可塑性の誘導(大脳皮質神経系)、(3)長期的な遺伝子発現制御(心筋細胞市神経細胞両者への分化能を有するP19細胞系)という3つの視点から系統的に調べる。

Research Products

• [Journal Article] Spontaneous Calcium Transients in Cultured Cortical Networks During Development (2009)
  • Author(s): Takayama Y, Moriguchi H, Kotani K, Jimbo Y.
  • Journal Title: IEEE Trans.BME 56 Pages: 2949-2956
  • Peer Reviewed
• [Journal Article] Light-Addressed Stimulation Under Ca^<2+> Imaging of Cultured Neuron (2009)
  • Author(s): Suzurikawa J, Nakao M, Jimbo Y, Takahashi H.
  • Journal Title: IEEE Trans.BME 56 Pages: 2660-2665
  • Peer Reviewed
• [Journal Article] Ensemble Stimulation of Embryoid Bodies using Substrate-Embedded Electrodes (2009)
  • Author(s): Takayama Y, Moriguchi H, Saito A, Jimbo Y.
  • Journal Title: IEEJ Trans. 4 Pages: 734-735
  • Peer Reviewed
• [Journal Article] Postural-induced phase shift of respiratory sinus arrhythmia and blood pressure variations-insight from respiratory-phase domain analysis (2008)
  • Author(s): Kotani K., Takamasu K., Jimbo Y, Yamamoto Y.
  • Journal Title: Am.J.Physiol. 294 Pages: H1481-H1489
  • Peer Reviewed
• [Journal Article] MEA-based recording of neuronal activity in vitro (2007)
  • Author(s): Jimbo Y.
  • Journal Title: Arch.Ita.Biol. 145 Pages: 289-297
  • Peer Reviewed
• [Presentation] Developmental Effects of Low Frequency Magnetic Fields on P19-Derived Neuronal Cells (2009)
  • Author(s): Saito A., Takayama Y., Moriguchi H., Kotani K., Jimbo Y.
  • Organizer: 31^<st> Ann. Int. IEEE EMBS Conf.
  • Place of Presentation: Minneapolis
  • Year and Date: 2009-09-02
• [Presentation] Ensemble Stimulation of Embryoid Bodies using microfabricated ITO Substrates (2009)
  • Author(s): Takayama Y., Moriguchi H., Saito A., Kotani K., Jimbo Y.
  • Organizer: 31^<st> Ann. Int. IEEE EMBS Conf.
  • Place of Presentation: Minneapolis
  • Year and Date: 2009-09-02
• [Presentation] Hierarchical oscillatory patterns observed in the spontaneous and evoked activity in cultured small recurrent networks (2008)
  • Author(s): Moriguchi H., Tamai N., Takayama Y., Kotani K., Jimbo Y.
  • Organizer: 6th FENS Forum
  • Place of Presentation: Geneva
  • Year and Date: 2008-07-12
• [Presentation] Neurons derived from P19 embryonal carcinoma cells establish functional neuronal network (2008)
  • Author(s): Takayama Y., Saito A., Moriguchi H., Kotani K., Jimbo Y.
  • Organizer: 6th FENS Forum
  • Place of Presentation: Geneva
  • Year and Date: 2008-07-12
• [Presentation] Characterization of Response Patterns Evoked by Light Addressed Electrical Stimulation in Cultured Neuronal Network (2008)
  • Author(s): Suzurikawa J. Nakao M., Jimbo Y., Kanzaki R., Takahashi H.
  • Organizer: 6th Int. Meet. Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen
  • Year and Date: 2008-07-08
• [Book] 分化誘導細胞によって形成した神経回路の電気活動(神原, 松永, 植田編)「シングルセル解析の最前線」分担執筆 (2010)
  • Author(s): 神保, 高山
  • Total Pages: 88-94
  • Publisher: CMC出版
• [Book] 神経回路活動の計測(合原, 神崎編)「理工系からの脳科学入門」分担執筆 (2008)
  • Author(s): 神保
  • Total Pages: 147-161
  • Publisher: 東京大学出版会
• [Book] 電極アレイシステムを用いる神経機能解析(酒井, 民谷編)「動物実験代替のためのバイオマテリアル・デバイス」分担執筆 (2007)
  • Author(s): 神保, 森口, 高山
  • Total Pages: 31-38
  • Publisher: CMC出版