2009 Fiscal Year Final Research Report
Regulation of Stem Cells Gene Expression using Apatite nanocarriers coated with Cell-reconizable chimeric Protein.
| Project/Area Number |
19200038
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
AKAIKE Toshihiro Tokyo Institute of Technology, フロンティア研究センター, 教授 (30101207)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 薬物伝達システム再生医療 / 炭酸アパタイト / ナノキャリアー / 細胞認識性バイオマテリアル / ES細胞 |
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| Research Abstract |
Targeted genes and relatives DNA RNA protein and drug involved delivery has received much attention throughout the world. In this study, we show for the first time pH-sensitive delivery of gene and relatives based on biocompatible inorganic nanoparticles of carbonate apatite being highly stable at the typical physiological pH and quickly degradable at the typical pH of endosomal compartment. This nano sized (100-300nm) apatite-gene conjugates demonstrated huge uptake of drug to various cells as determined by Florescence microscopy and flow cytometry. The application of carbonate apatite nanocarriers systems to stem-cells (ES, EC Cells) are successfully done with aid of cell-recogonizable nano-biomaterials such as chimeric antibodies as well as sugar-carrying polymers design.
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