Isotopomer Analyses of biophile elements in microbial greenhouse gases using incubation experiments.

2009 Fiscal Year Final Research Report

• Project/Area Number: 19201004
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental dynamic analysis
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: YOSHIDA Naohiro Tokyo Institute of Technology, 大学院・総合理工学研究科, 教授 (60174942)
• Co-Investigator(Kenkyū-buntansha): TOYODA Sakae 東京工業大学, 大学院・総合理工学研究科, 助教 (30313357) ; YAMADA Keita 東京工業大学, 大学院・総合理工学研究科, 助教 (70323780) ; UENO Yuuichiro 東京工業大学, グローバルエッジ研究院, テニュア・トラック助教 (90422542) ; KOBA Keisuke 東京農工大学, 大学院・共生科学技術研究院, 特任准教授 (90311745)
• Project Period (FY): 2007 – 2009
• Keywords: 物質循環 / 安定同位体 / 微生物 / ガス代謝

Research Abstract

Changes in atmospheric concentrations of greenhouse gases (GHGs) and aerosols are drivers of climate change. N_2O and CH_4 are important GHGs and H_2S is related to aerosol formation. To develop the source-sink analyses of these gases using their isotopomer signals, we quantified relationships between some microbial processes and their isotopomer signals in pure culture and co-culture conditions.

Research Products

• [Journal Article] The source of nitrous oxide emission from cattle manure composting process revealed by isotopomer analysis of and amoA abundance in beta-proteobacterial ammonia-oxidizing bacteria. (2010)
  • Author(s): Maeda, K., S. Toyoda, N. Yoshida
  • Journal Title: Applied and Environmental Microbiology 76(5) Pages: doi: 10. 1128/AEM. 01394-09
  • Peer Reviewed
• [Journal Article] Contribution of atmospheric nitrate to stream-water nitrate in Japanese coniferous forests revealed by oxygen isotope ratio of nitrate (2010)
  • Author(s): Tobari, Y., K. Koba, S. Toyoda, N. Yoshida
  • Journal Title: Rapid Communications in Mass Spectrometry 24 Pages: 1-6 doi: 10. 1002/rcm. 4498
  • Peer Reviewed
• [Journal Article] Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan (2010)
  • Author(s): Kimura, H., H. Nashimoto, M. Shimizu, S. Hattori, K. Yamada, K. Koba, N. Yoshida, K. Kato
  • Journal Title: The ISME Journal 4 Pages: 531-541
  • Peer Reviewed
• [Journal Article] Biomass production and energy source of thermophiles in a Japanese alkaline geothermal pool (2009)
  • Author(s): Kimura, H., K. Mori, H. Nashimoto, S. Hattori, K. Yamada, K. Koba, N. Yoshida, K. Kato
  • Journal Title: Environmental Microbiology 12(2) Pages: 480-489, dol: 10. 1111/ j. 1462-2920
  • Peer Reviewed
• [Journal Article] Isotopomeric analysis of N2O dissolved in a river in the Tokyo metropolitan area (2009)
  • Author(s): Toyoda, S., H. Iwai, K. Koba, N. Yoshida
  • Journal Title: Rapid Communications in Mass Spectrometry 23 Pages: 809-821, doi: 10. 1002/rcm. 3945
  • Peer Reviewed
• [Journal Article] Biogeochemistry of nitrous oxide in groundwater in a forested ecosystem elucidated by nitrous oxide isotopomer measurements. (2009)
  • Author(s): Koba, K, S. Toyoda, N. Yoshida
  • Journal Title: Geochimica et Cosmochimica Acta 73 Pages: 3115-3133
  • Peer Reviewed
• [Journal Article] A 6. 5-year continuous record of sea surface salinity and seawater isotopic composition at Harbor of Ishigaki Island, southwest Japan (2009)
  • Author(s): Abe, O., N. Yoshida
  • Journal Title: Isotopes in Environmental and Health Studies 45(3) Pages: 247-258
  • Peer Reviewed
• [Journal Article] Denitrification and nitrous oxide cycling within the upper oxycline of the oxygen minimum zone off the eastern tropical South Pacific (2009)
  • Author(s): Farias, L., N. Yoshida
  • Journal Title: Limnology and Oceanography 54(1) Pages: 132-144
  • Peer Reviewed
• [Journal Article] Quadruple sulfur isotope analysis of ca. 3. 5 Ga Dresser Formation: new evidence for microbial sulfate reduction in the Early Archean (2008)
  • Author(s): Ueno, Y., S. Ono, D. Rumble, S. Maruyama
  • Journal Title: Geochimica et Cosmoshimica Acta 72 Pages: 5675-5691
  • Peer Reviewed
• [Journal Article] Role of nitrification and denitrification on the nitrous oxide in the eastern tropical North Pacific and Gulf of California (2007)
  • Author(s): Yamagishi, H., S. Toyoda, N. Yoshida, K. Koba, Y. Yamanaka
  • Journal Title: Journal of Geophysical Research-Biogeosciences 112 Pages: G02015
  • Peer Reviewed
• [Journal Article] Nitrogen Isotope Study on Nitrate-Contaminated Groundwater in the Sichuan Basin, China (2007)
  • Author(s): Li, X., H. Masuda, K. Koba, H. Zeng
  • Journal Title: Water, Air and Soil Pollution 178 Pages: 145-156
  • Peer Reviewed
• [Journal Article] Possible mechanisms leading to a delay in carbon stock recovery after land use change (2007)
  • Author(s): Arai H, N. Tokuchi, K. Koba
  • Journal Title: Soil Science Society of America Journal 71 Pages: 1636-1638
  • Peer Reviewed
• [Presentation] 自然安定同位体比を用いた一酸化二窒素生成プロセスの解析 (2009)
  • Author(s): 木庭啓介
  • Organizer: 微生物生態学会
  • Place of Presentation: 西条
  • Year and Date: 2009-11-21
• [Presentation] アイソトポマー比を用いた農耕土壌起源N2Oのキャラクタリゼーションおよび全球放出推定 (2009)
  • Author(s): 豊田栄、木庭啓介、山田桂太、吉田尚弘
  • Organizer: 第15回大気化学討論会
  • Place of Presentation: つくば
  • Year and Date: 2009-10-21
• [Presentation] Investigation of methane emission during sewage watger treatment in Japan throughcarbon isotope ratio (2009)
  • Author(s): Wu, C., K. Yamada, S. Toyoda, N. Yoshida, R. Kono, K. Murayama
  • Organizer: 日本地球化学会第56回年会
  • Place of Presentation: 西条
  • Year and Date: 2009-09-17
• [Presentation] Characterizing the methane cycle in lake system using isotopic signature in Lake-Kizaki, Japan (2009)
  • Author(s): Lombard, L., K. Yamada, S. Hattori, Y. Sasaki, A. Makabe, K. Koba, N. Yoshida
  • Organizer: 日本地球化学会第56回年会
  • Place of Presentation: 西条
  • Year and Date: 2009-09-16
• [Presentation] Quadruple Sulfur Isotope Analysis of Sulfur Cycle in a Stratified Lake (2009)
  • Author(s): Lombard, L., K. Yamada, S. Hattori, Y. Sasaki, A. Makabe, K. Koba, N. Yoshida
  • Organizer: Goldschmidt Conference
  • Place of Presentation: Davos, Switzerland
  • Year and Date: 2009-06-23
• [Presentation] 家畜排泄物堆肥化におけるN2O生成機構の安定同位体解析 (2009)
  • Author(s): 下島涼介、豊田栄、吉田尚弘、前田高輝、長田隆
  • Organizer: 日本地球惑星科学連合大会
  • Place of Presentation: 千葉
  • Year and Date: 2009-05-20
• [Presentation] isotope fractionation by methane production in co-culture between fermenters and hydrogenotrophic methanogens. (2009)
  • Author(s): Hattori, S., H. Kimura, H. Nashimoto, K. Koba, K. Yamada, M. Shimizu, H. Watanabe, M. Yoh, N. Yoshida. Carbon, hydrogen
  • Organizer: 日本地球惑星科学連合大会
  • Place of Presentation: 千葉
  • Year and Date: 2009-05-20
• [Presentation] Stable isotope and microbial analyses of methane-producing process in a geothermal aquifer associated with the subsurface of the accretionary prism (2009)
  • Author(s): Hattori, S., H. Kimura, H. Nashimoto, K. Koba, K. Yamada, M. Shimizu, H. Watanabe, M. Yoh, N. Yoshida.
  • Organizer: Japan, EGU General Assembly
  • Place of Presentation: Vienna.
  • Year and Date: 2009-04-21
• [Presentation] sotopomeric analysis of N2O emitted from temperate agricultural soils (2008)
  • Author(s): Toyoda, S., K. Koba, K. Yamada, N. Yoshida
  • Organizer: the Fourth International Symposium on Isotopomers
  • Place of Presentation: Tokyo
  • Year and Date: 20081004-20081008
• [Presentation] Biological fractionations of quadruple sulfur isotopes in a stratified lake (2008)
  • Author(s): Nakagawa, M., Y. Ueno, N. Yoshida
  • Organizer: the Fourth International Symposium on Isotopomers
  • Place of Presentation: Tokyo
  • Year and Date: 20081004-20081008
• [Presentation] 下水高度処理過程における溶存態メタンの安定同位体を用いた解析 (2008)
  • Author(s): 服部祥平、山田桂大、豊田栄、鈴木有理、藤井彩子、吉田尚弘、河野里名、村山康樹
  • Organizer: 日本地球化学会第55回年会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-17
• [Presentation] Biological fractionations of quadruple sulfur isotopes in a stratified lake (2008)
  • Author(s): Ueno, Y., M. Nakagawa, N. Yoshida
  • Organizer: Goldschmidt Conference
  • Place of Presentation: Vancouver, Canada.
  • Year and Date: 2008-07-14
• [Presentation] 培養実験による硫黄還元菌の四種硫黄同位体分別効果の決定 (2007)
  • Author(s): 多田敦志、上野雄一郎、高井研、吉田尚弘
  • Organizer: 日本地球化学会第53回年会
  • Place of Presentation: 岡山
  • Year and Date: 2007-09-20
• [Presentation] 、施肥された農耕土壌から放出されるN2Oのアイソトポマー比 (2007)
  • Author(s): 矢野翠、豊田栄、木庭啓介、吉田尚弘
  • Organizer: 日本地球化学会第53回年会
  • Place of Presentation: 岡山
  • Year and Date: 2007-09-19
• [Presentation] 牛ふん堆肥化過程で発生するN2Oのアイソトポマー比 (2007)
  • Author(s): 深澤真、豊田栄、前田高輝、長田隆、白石誠、吉田尚弘
  • Organizer: 日本地球化学会第53回年会
  • Place of Presentation: 岡山
  • Year and Date: 2007-09-19
• [Book] からだと酸素の事典、地球の酸素環境の歴史 (2009)
  • Author(s): 田村守, 他、執筆者:吉田尚弘, 他
  • Total Pages: 13P(2P～14P)
  • Publisher: 朝倉書店
• [Book] からだと酸素の事典、地球温暖化と酸素環境の未来 (2009)
  • Author(s): 田村守, 他、執筆者:吉田尚弘, 他
  • Total Pages: 5P(56P～60P)
  • Publisher: 朝倉書店
• [Remarks] ホームページ等
  • URL: http://nylab.chemenv.titech.ac.jp/