Application of ion channel biosensor to single neural cell analysis

2010 Fiscal Year Final Research Report

• Project/Area Number: 19201028
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Microdevices/Nanodevices
• Research Institution: Institute for Molecular Science
• Principal Investigator: URISU Tsuneo Institute for Molecular Science, 生命・錯体分子科学研究領域, 教授 (50249950)
• Co-Investigator(Kenkyū-buntansha): MAO Yanli 分子科学研究所, 生命・錯体分子科学研究領域, 特別協力研究員 (80442531) ; KIM Yonghoon 分子科学研究所, 生命・錯体分子科学研究領域, 特別協力研究員 (80509852)
• Co-Investigator(Renkei-kenkyūsha): TERO Ryugo 分子科学研究所, 生命・錯体分子科学研究領域, 助教 (40390679) ; ISHII Kiyoshi 中部大学, 工学部, 准教授 (70410644) ; ASANO Toshifumi 分子科学研究所, 生命・錯体分子科学研究領域, 特別協力研究員 (30552476) ; NAGAHIRO Takeshi 分子科学研究所, 生命・錯体分子科学研究領域, 特別協力研究員 (40585113)
• Project Period (FY): 2007 – 2010
• Keywords: プレーナーパッチクランプ / 光感受性イオンチャンネル / 神経細胞ネットワーク / マイクロ流路 / ChR2 / ホットエンボス

Research Abstract

We have developed the elementary technology necessary to apply the planer patch clamp technology to the single neural cell analysis. Incubation type planar patch clamp device was developed and (1) Ligand gated ion channel current measurement by whole cell mode was attained, (2) Transfection of photosensitive ion channel to HEK293 and cloning were attained, (3) Neural network was formed and photosensitive ion channel was transfected, (4) Highly stable electrode of planar patch clamp was developed, and (5) Generation of active potential by laser irradiation was attained. All necessary elementary technologies were successfully developed.

Research Products

• [Journal Article] Clustering effects of GM1 and formation mechanisms of interdigitated liquid disordered domains in GM1/SM/CHOL-supported planar bilayers on mica surface (2010)
  • Author(s): Zhiguo Shang, Yanli Mao, Ryugo Tero, Tyuji Hoshino, Motohiko Tanaka, Tsuneo Urisu
  • Journal Title: Chem.Phys.Lett 497 Pages: 108-114
  • Peer Reviewed
• [Journal Article] The PDMS-based microfluidic channel fabricated by synchrotron radiation stimulated etching (2010)
  • Author(s): Tingchao He, Changshun Wang, Tsuneo Urisu, Takeshi Nagahiro, Ryugo Tero, Rong Xia
  • Journal Title: Optics Express 18 Pages: 9733-9738
  • Peer Reviewed
• [Journal Article] Surface-induced phase separation of a sphingomyelin/cholesterol/ganglioside GM1-planar bilayer on mica surfaces and microdomain molecular conformation that accelerate Aβ oligomerization (2010)
  • Author(s): Yanli Mao, Zhiguo Shang, Yosuke Imai, Tyuji Hoshino, Ryugo Tero, Motohiko Tanaka, Naoki Yamamoto, Katsuhiko Yanagisawa, Tsuneo Urisu
  • Journal Title: BBA Biomembranes 1798 Pages: 1090-1099
  • Peer Reviewed
• [Journal Article] Synchrotron-radiation-stimulated etching of polydimethylsiloxane (PDMS) using XeF_2 as a reaction gas (2010)
  • Author(s): Tsungyi Chiang, Tetsuya Makimura, Tingchao He, Shuichi Torii, Tomoko Yoshida, Ryugo Tero, Changshun Wang, Tsuneo Urisu
  • Journal Title: J.Synchrotron Rad. 17 Pages: 69-74
  • Peer Reviewed
• [Journal Article] Shape Transformation of Adsorbed Vesicles on Oxide Surfaces : Effect of Substrate Material and Photo-Irradiation (2009)
  • Author(s): R.Tero, T.Ujihara, T.Urisu
  • Journal Title: Trans.Mater.Res.Soc.Jpn. 34 Pages: 183-188
  • Peer Reviewed
• [Journal Article] Noise Property of Incubation Type Planar Ion Channel Biosensor (2009)
  • Author(s): Toshifumi Asano, Takuya Nakamura, Akihiro Wakahara, Tsuneo Urisu
  • Journal Title: Jpn.J.Appl.Phys. 48 Pages: 027001-1-4
  • Peer Reviewed
• [Journal Article] New Infrared reflection absorption spectroscopy (IRRAS) system for observation of solid-solution interface biomaterials (2008)
  • Author(s): Md.Abu Sayed, Hidetaka Uno, Kensuke Harada, Keiichi Tanaka, Yong-Hoon Kim, Yuichiro Nakaoki, Koji Okumura, Ryugo Tero, Tsuneo Urisu
  • Journal Title: Chem Phys.Lett. 466 Pages: 235-239
  • Peer Reviewed
• [Journal Article] Lipid Bilayer Membrane with Atomic Step Structure : Supported Bilayer on a Step-and-Terrace TiO_2(100) Surface (2008)
  • Author(s): Ryugo Tero, Toru Ujihara, Tsuneo Urisu
  • Journal Title: Langmuir 24 Pages: 11567-11576
  • Peer Reviewed
• [Journal Article] The morphology of GM1_x/SM_<0.6-x>/Cho1_<0.4> planar bilayers supported on SiO_2 surfaces (2008)
  • Author(s): Yanli Mao, Ryugo Tero, Yosuke Imai, Tyuji Hoshino, Tsuneo Urisu
  • Journal Title: Chem.Phys.Lett. 460 Pages: 289-294
  • Peer Reviewed
• [Journal Article] Incubation type Si-based planer ion channel biosensor (2008)
  • Author(s): Tsuneo Urisu, Toshifumi Asano, Zhenlong Zhang, Hidetaka Uno, Ryugo Tero, Han Junkyu, Isoda Hiroko, Yusuke Arima, Hiroo Iwata, Koji Shibasaki, Makoto Tominaga
  • Journal Title: Analytical and Bioanalytical Chemistry 391 Pages: 2703-2709
  • Peer Reviewed
• [Journal Article] A Cell Culture Type Planar Ion-Channel Biosensor (2008)
  • Author(s): Toshifumi Asano, Hidetaka Uno, Koji Shibasaki, Makoto Tominaga, Tsuneo Urisu
  • Journal Title: Transaction of Materials Research Society 33 Pages: 767-770
  • Peer Reviewed
• [Journal Article] Supported lipid bilayers membranes on SiO2 and TiO_2 : Substrate effects on membrane formation and shape transformation (2007)
  • Author(s): R.Tero, T.Ujihara, T.Urisu
  • Journal Title: Proc.of SPIE 6769 Pages: 67690J
  • Peer Reviewed
• [Journal Article] Fabrication of a planar type patch-clamp biosensor using silicon on insulator substrate (2007)
  • Author(s): Toshifumi Asano, Zheng Long Zhang, Hidetaka Uno, Ryugo Tero, Koichi Suzui, Satoshi Nakao, Takashi Kaito, Koji Shibasaki, Makoto Tominaga, Yuichi Utsumi, Tsuneo Urisu
  • Journal Title: J.Surf.Sci.Society Japan. 28 Pages: 385-390
  • Peer Reviewed
• [Journal Article] Fabrication of Si-based Planar Type Patch-Clamp Biosensor Using SOI Substrate (2007)
  • Author(s): Z.L.Zhang, T.Asano, H.Uno, R.Tero, M.Suzui, S.Nakao, T.Kaito, K.Shibasaki, M.Tominaga, Y.Utsumi, Y.L.Gao, T.Urisu
  • Journal Title: Thin Solid Films 516/9 Pages: 2831-2833
  • Peer Reviewed
• [Presentation] An in vitro neural network device as a useful research platform of neuro science and nanomedicine (2011)
  • Author(s): Tsune Urisu
  • Organizer: The 2^<nd> Taiwan-Japan Symposium on Nanomedicine
  • Place of Presentation: Academia Sinica, Taipei.
  • Year and Date: 20110224-20110225
• [Presentation] Development of planar patch-clamp type photostimulation neural cell network device (2010)
  • Author(s): T.Urisu
  • Organizer: Japan-Taiwan Symposium on Nano-Medicine Research and Education
  • Place of Presentation: Kyoto
  • Year and Date: 2010-01-25
• [Presentation] Development of Neural Network Devices as a New Methodology for Physical Chemistry Investigation of Neuroacience and Neurodegenerative Diseases (2009)
  • Author(s): T.Urisu
  • Organizer: The 2nd Japan-Korea Seminar on Biomolecular Sciences-Experiments and Simulations
  • Place of Presentation: Nagoya
  • Year and Date: 20091222-20091223
• [Presentation] Si based planer type ion-channel biosensor and its applications (2007)
  • Author(s): T.Urisu
  • Organizer: The 2007 International Conference on Solid State Devices and Materials
  • Place of Presentation: Tsukuba.
  • Year and Date: 20070919-20070921
• [Presentation] Development of Planer type ion-channel biosensor (2007)
  • Author(s): T.Urisu
  • Organizer: The 5^<th> International Forum of Post-Genome Technologies (5'IFPT)
  • Place of Presentation: Suzhou, China.
  • Year and Date: 20070910-20070911
• [Patent(Industrial Property Rights)] 神経細胞機能解析素子およびイオンチャンネル電流の測定方法 (2010)
  • Inventor(s): 宇理須恒雄、鈴井光一、青山正樹、高田紀子、王志宏、宇野秀隆
  • Industrial Property Rights Holder: 大学共同利用機関法人自然科学研究機構
  • Industrial Property Number: 特許出願,2010-204326
  • Filing Date: 2010-09-13