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2008 Fiscal Year Final Research Report

An Integrated Strategy for Unveiling the Function of KAONASHI Unknown (Functional) Genes

Research Project

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Project/Area Number 19201038
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 基礎ゲノム科学
Research InstitutionKeio University

Principal Investigator

SHIMIZU Nobuyoshi  Keio University, 医学部, 名誉教授 (50162706)

Co-Investigator(Kenkyū-buntansha) ASAKAWA Shuichi  慶應義塾大学, 医学部, 講師 (30231872)
SHIMZU Atsushi  慶應義塾大学, 医学部, 助教 (30327655)
SASAKI Takashi  慶應義塾大学, 医学部, 助教 (70306843)
Research Collaborator YO Go  慶應義塾大学, 医学部, 助教 (10464992)
SHIOHAMA Aiko  慶應義塾大学, 医学部, 助教 (40383731)
KOJIMA Sabinukazuko  慶應義塾大学, 医学部, 研究員 (10445439)
Project Period (FY) 2007 – 2008
Keywordsメダカ / ノックダウン / モルフォリノ / カオナシ
Research Abstract

カオナシ遺伝子を統合的に解析するために、慶應ヒト遺伝子データベースを構築し、メダカカオナシ遺伝子のノックダウン解析結果や初期胚における発現パターンによる検索を可能とした。さらにヒトカオナシ遺伝子を培養細胞で強制発現させた細胞内局在解析や、高効率なメダカ発現コンストラクトの開発を行った。タンパク質コード遺伝子のみならず小分子カオナシRNAを対象としてシーケンシングと解析を行った。

  • Research Products

    (13 results)

All 2010 2009 2008 2007 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Retinoic Acid Signaling Positively Regulates Liver Specification by Inducing wnt2bb Gene Expression in Medaka2010

    • Author(s)
      Negishi, T., Nagai, Y., Asaoka, Y., Namae, M., Mitani, H., Sasaki, T., Shimizu, N., Terai, S., Sakaida, I., Kondoh, H., Katada, T., Furutani-Seiki, M., Nishina, H.
    • Journal Title

      Hepatol 51(3)

      Pages: 1037-1045

    • Peer Reviewed
  • [Journal Article] Two distinct types of theca cells in the medaka gonad : germ cell-dependent maintenance of cyp19al-expressing theca cells2009

    • Author(s)
      Nakamura, S., Kurokawa, H., Asakawa, S., Shimizu, N., Tanaka M.
    • Journal Title

      Dev Dyn 238(10)

      Pages: 2652-2657

    • Peer Reviewed
  • [Journal Article] Ethylnitrosourea-induced thymus-defective mutants identify roles of KIAA1440, TRRAP, and SKIV2L2 in teleost organ development2009

    • Author(s)
      Iwanami, N., Okada, M., Hoa, V. Q., Seo, Y., Mitani, H., Sasaki, T., Shimizu. N., Kondoh, H., Furutani-Seiki, M., Takahama, Y.
    • Journal Title

      Eur J Immunol 39(9)

      Pages: 2606-2616

    • Peer Reviewed
  • [Journal Article] Polycystic Kidney Disease in the Medaka (Oryzias latipes) pc Mutant Caused by a Mutation in the Gli-Similar3 (glis3) Gene.2009

    • Author(s)
      Hashimoto, H., Miyamoto, R., Watanabe, N., Shiba, D., Ozato, K., Inoue, C., Kubo, Y, Koga, A., Jindo, T., Narita, T., Naruse, K., Ohishi, K., Nogata, K., Shin-I, T., Asakawa, S., Shimizu. N., Miyamoto, T., Mochijiki, T., Yokoyama, T., Hori, H., Takeda, T., Kohara, Y., Wakamatsu, Y.
    • Journal Title

      Plos ONE 4(7)

      Pages: e6299

    • Peer Reviewed
  • [Journal Article] Cell Cycle Progression, and Teleost Organ Development2008

    • Author(s)
      Iwanami, N., Higuchi, T., Sasano, Y., Fujiwara, T., Hoa, V. Q., Okada, M., Talukder, S. R., Kunimatsu, S., Li, J., Saito, F., Bhattacharya, C., Matin, A., Sasaki, T., Shimizu. N., Mitani, H., Himmelbauer, H., Momoi, A., Kondoh, H., Furutani-Seiki, M., Takahama, Y.
    • Journal Title

      PLoS Genet 4(8)

      Pages: e1000171

    • Peer Reviewed
  • [Journal Article] Myocyte enhancer factor 2 regulates expression of medaka Oryzias latipes fast skeletal myosin heavy chain genes in a temperature-dependent manner2008

    • Author(s)
      Liang, C. S., Ikeda, D., Kinoshita, S., Shimizu, A., Sasaki, T., Asakawa, S., Shimizu. N., Watabe, S.
    • Journal Title

      Gene 407

      Pages: 42-53

    • Peer Reviewed
  • [Journal Article] UTGB/medaka : Genomic Resource Database for Medaka Biology2008

    • Author(s)
      Ahsan, B., Kobayashi, D., Yamada, T., Kasahara, M., Sasaki, S., Saito, T. L., Nagayasu, Y., Doi, K., Nakatani, Y, Qu, W., Jindo, T., Shimada, A., Naruse, K., Toyoda, A., Kuroki, Y., Fujiyama, A., Sasaki, T., Shimizu, A., Asakawa, S., Shimizu. N., Hashimoto, S. I., Yang, J., Lee, Y. Matsushima, K., Sugano, S., Sakaizumi M, Narita T, Ohishi K, Haga S, Ohta F, Nomoto H, Nogata K, Morishita, T., Endo, T., Shin-I., T., Takeda, H., Kohara, Y., Morishita, S.
    • Journal Title

      Nucleic Acids Res. 36

      Pages: D747-752

    • Peer Reviewed
  • [Journal Article] Radiation hybrid maps of Medaka chromosomes LG 12, 17, and 222007

    • Author(s)
      Su, F., Osada, Y., Ekker, M., Chevrette, M., Shimizu, A., Asakawa, S., Shiohama, A., Sasaki, T., Shimizu. N., Yamanaka, T., Sasado, T., Mitani, H., Geisler, R., Kondoh, H., Furutani-Seiki, M.
    • Journal Title

      DNA Res 14(3)

      Pages: 135-40

    • Peer Reviewed
  • [Journal Article] The hotei mutation of medaka in the anti-Mullerian hormone receptor causes the dysregulation of germ cell and sexual development2007

    • Author(s)
      Morinaga, C., Saito, D., Nakamura, S., Sasaki, T., Asakawa, S., Shimizu, N., Mitani, H., Furutani-Seiki, M., Tanaka, M., Kondoh, H.
    • Journal Title

      PNAS. 104(23)

      Pages: 9691-9696

    • Peer Reviewed
  • [Presentation] カオナシ遺伝子群の解析およびオンライン画像データベースの構築2008

    • Author(s)
      浅川修一, 清水厚志, 佐々木貴史, 清水信義
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      (神戸ポートアイランド) 神戸
    • Year and Date
      2008-12-12
  • [Presentation] カオナシ遺伝子群のノックダウン解析およびオンラインデータベースの構築2007

    • Author(s)
      清水厚志, 浅川修一, 佐々木貴史, 清水信義
    • Organizer
      第30回日本分子生物学会年会
    • Place of Presentation
      (パシフィコ横浜) 横浜
    • Year and Date
      2007-12-11
  • [Presentation] The American Society of Human Genetics 57th Annual Meeting2007

    • Author(s)
      Shimizu, A, Asakawa, S., Sasaki, T., Shimizu. N., An On-line Database System for Knock-down Analysis of KAO-NASHI Genes
    • Organizer
      San Diego
    • Year and Date
      2007-10-26
  • [Remarks] N-K高校講座生物第23回 第3部 遺伝 20071015

    • URL

      http://www.nhk.or.jp/kokokoza/tv/seibutsu/archive/resume023.htm

URL: 

Published: 2011-06-18   Modified: 2016-04-21  

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