2010 Fiscal Year Final Research Report
Creation of highly-functional nucleic acids by unnatural base pair systems
| Project/Area Number |
19201046
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
HIRAO Ichiro 独立行政法人理化学研究所, 核酸合成生物学研究チーム, チームリーダー (50173216)
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| Co-Investigator(Renkei-kenkyūsha) |
KIMOTO Michiko 独立行政法人理化学研究所, 核酸合成生物学研究チーム, 研究員 (20415144)
MITSUI Tsuneo 独立行政法人理化学研究所, 核酸合成生物学研究チーム, 客員研究員 (60345155)
KAWAI Rie 独立行政法人理化学研究所, 核酸合成生物学研究チーム, リサーチアソシエイト (10415135)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 人工塩基対 / 核酸アプタマー / PCR / in vitroセレクション / SELEX |
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| Research Abstract |
By using our unnatural base pair systems for expanding the genetic alphabet of DNA, we developed a new method to generate artificial nucleic acid molecules with increased functionality. Here, we succeeded in an evolutional engineering method(in vitro selection, SELEX) generating new DNA aptamers, which comprise one unnatural and four natural bases. The aptamers obtained here exhibited higher affinity to target proteins than those of conventional ones.
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Research Products
(35 results)
