2010 Fiscal Year Final Research Report
Development of functional anti-cancer molecules based on precise control of excitation and relaxation pathways
| Project/Area Number |
19205021
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
URANO Yasuteru The University of Tokyo, 大学院・医学系研究科, 教授 (20292956)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OKUNO Hiroyuki 東京大学, 大学院・医学系研究科, 助教 (80272417)
|
|---|
| Project Period (FY) |
2007 – 2010
|
| Keywords | 蛍光プローブ / 分子イメージング / がん / 光誘起電子移動 / エンドサイトーシス / リソソーム / ペプチダーゼ / 光線力学的治療 |
|---|
| Research Abstract |
It is a long-term goal of cancer diagnosis to develop tumor-imaging techniques that have sufficient specificity and sensitivity. To achieve this goal, minimizing the background signal originating from non-target tissues is critical. Here, we achieve highly specific in vivo cancer visualization by employing a newly-designed targeted "activatable" fluorescent imaging probe. This agent is activated after cellular internalization by sensing the pH change in the lysosome. Novel acidic pH-activatable probes based on the BODIPY fluorophore were synthesized, and then conjugated to a cancer-targeting monoclonal antibody. As proof of concept, ex and in vivo imaging of HER2-positive lung cancer cells in mice were performed. The probe was highly specific for tumors with minimal background signal.
|
-
-
[Journal Article] Selective molecular imaging of viable cancer cells with pH-activatable fluorescence probes.2009
Author(s)
Urano Y, Asanuma D, Hama Y, Koyama Y, Barrett T, Kamiya M, Nagano T, Watanabe T, Hasegawa A, Choyke PL, Kobayashi H
-
Journal Title
Nat.Med. 15
Pages: 104-109
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
