2010 Fiscal Year Final Research Report
Development of analytical and production method for problematic protein by artificial control of physical property of protein
| Project/Area Number |
19206085
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Okayama University |
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Principal Investigator |
YAMADA Hidenori Okayama University, 大学院・自然科学研究科, 教授 (80037613)
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| Co-Investigator(Kenkyū-buntansha) |
FUTAMI Jyunichirou 岡山大学, 大学院・自然科学研究科, 准教授 (00420498)
SENOO Masaharu 岡山大学, 大学院・自然科学研究科, 教授 (90243493)
TADA Hiroko 岡山大学, 自然生命科学研究支援センター, 准教授 (60271061)
KOSAKA Megumi 岡山大学, 自然生命科学研究支援センター, 助手 (00170233)
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| Project Period (FY) |
2007 – 2010
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| Keywords | バイオテクノロジー / 生物・生体工学 / 蛋白質 / 化学修飾 / 結晶化 / 可溶化 |
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| Research Abstract |
This study aimed development of handling technology especially for problematic protein using chemical conjugation techniques and protein crystallization methodology. Novel chemical modification reagent and optimization of protein preparation techniques provided improved transduction of transcription factor protein into cells, and solubilization of total cellular protein from cancer cells. Together with a newly developed protein crystallization tag sequence, these technologies will give a useful methodology for structure and functional analysis of proteins and their medical applications.
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[Journal Article] Intracellular delivery of glutathione S-transferase-fused proteins into mammalian cells by polyethylenimine-glutathione conjugates.2008
Author(s)
Hitoshi Muratal, Junichiro Futami, Midori Kitazoe, Takayuki Yonehara, Hidetaka Nakanishi, Megumi Kosaka, Hiroko Tada, Masakiyo Sakaguchi, Yasuyuki Yagi, Masaharu Seno, Nam-ho Huh, Hidenori Yamada.
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Journal Title
Journal of Biochemistry 144(4)
Pages: 447-455
Peer Reviewed
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[Journal Article] 'Crystal lattice engineering,' an approach to engineer protein crystal contacts by creating intermolecular symmetry : crystallization and structure determination of a mutant human RNase 1 with a hydrophobic interface of leucines.2007
Author(s)
Yamada H., Tamada T., Kosaka M., Miyata K., Fujiki S., Tano M., Moriya M., Yamanishi M., Honjo E., Tada H., Ino T., Yamaguchi H., Futami J., Seno M., Nomoto T., Hirata T., Yoshimura M., Kuroki R.
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Journal Title
Protein Sci. 16
Pages: 1389-1397
Peer Reviewed
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