Post-transcriptional regulation associated with RNA modifications responsible for higher order biological processes

2009 Fiscal Year Final Research Report

• Project/Area Number: 19207013
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: SUZUKI Tsutomu The University of Tokyo, 大学院・工学系研究科, 教授 (20292782)
• Project Period (FY): 2007 – 2009
• Keywords: RNA修飾 / RNAエディティング / イノシン / メチル化 / piRNA

Research Abstract

This project aimed to study post-transcriptional regulation associated with RNA modifications responsible for higher order biological processes. We found that piwi-interacting RNAs (piRNA) expressed in mouse testis have 2'-O-methylation at their 3' termini, and characterized the methyltransferase responsible for this modification. Concerning mRNA modifications, we took notice on inosines that are abundantly introduced in RNAs from human brain. We have established a biochemical method, named "inosine chemical erasing (ICE)", to directly identify inosines on RNA strands. We have mapped more than 16,000 novel sites of inosines in human brain transcriptome. Furthermore, we have started a project of genome-wide identification of inosine sites by using the ICE-method combined with the deep sequencing (ICE-Seq).

Research Products

• [Journal Article] Discovery and characterization of tRNAIle lysidine synthetase (Ti1S) (2010)
  • Author(s): Suzuki, T., Miyauchi, K.
  • Journal Title: FEBS Lett. 584 Pages: 272-277
• [Journal Article] Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA (2010)
  • Author(s): Kimura, S., Suzuki, T.
  • Journal Title: Nucleic Acids Res. 38 Pages: 1341-1352
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline Germline (2009)
  • Author(s): Shoji, M., Tanaka, T., Hosokawa, M., Reuter, M., Stark, A., Kato, Y., Kondoh, G., Okawa, K., Chujo, T., Suzuki, T., Hata, K., Martin, S. L., Noce, T., Kuramochi-Miyagawa, S., Nakano, T., Sasaki, H., Pillai, R. S., Nakatsuji, N. Chuma, S.
  • Journal Title: Dev Cell 17 Pages: 775-787
• [Journal Article] Structural basis for translational fidelity ensured by tRNA lysidine synthetase (2009)
  • Author(s): Nakanishi, K., Bonnefond, L., Kimura, S., Suzuki, T., Ishitani, R., Nureki, O.
  • Journal Title: Nature 461 Pages: 1144-1148
• [Journal Article] 3D-structure of mammalian mitochondrial tRNAAsp revealed by solution probing and phylogeny (2009)
  • Author(s): Messmer, M., Putz, J., Suzuki, T., Suzuki T., Sauter, C., Sissler, M., Florentz, C.
  • Journal Title: Nucleic Acids Res. 37 Pages: 6881-6895
• [Journal Article] Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme, TYW2 (2009)
  • Author(s): Umitsu, M., Nishimasu, H., Noma, A., Suzuki, T., Ishitani, R., Nureki, O.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 106 Pages: 15616-15621
• [Journal Article] Biogenesis of glutaminyl-tRNAGIn in human mitochondria (2009)
  • Author(s): Nagao, A., Suzuki, T., Katoh, T., Sakaguchi, Y., Suzuki T.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 106 Pages: 16209-16214
• [Journal Article] The R336Q mutation in human mitochondrial EFTu prevents the formation of an active mt-EETu・GTP・aa-tRNA ternary complex (2009)
  • Author(s): Valente, L., Shigi, N., Suzuki, T., Zeviani, M.
  • Journal Title: Biochim Biophys Acta. 1792 Pages: 791-795
• [Journal Article] Aquifex aeolicus tRNA (N2, N2-guanine)-dimethyltransferase (Trml) catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA (2009)
  • Author(s): Awai, T., Kimura, S., Tomikawa, C., Ochi, A., Ihsanawati, Bessho, Y., Yokoyama, S., Ohno, S., Nishikawa, K., Yokogawa, T., Suzuki T., Hori, H.
  • Journal Title: J.Biol.Chem. 284 Pages: 20467-20478
• [Journal Article] The ordered transcription of RNA domains is not essential for ribosome biogenesis (2009)
  • Author(s): Kitahara, K., Suzuki, T.
  • Journal Title: Mol Cell. 34 Pages: 760-766
• [Journal Article] Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4 (2009)
  • Author(s): Suzuki, Y., Noma, A., Suzuki, T., Ishitani, R., Nureki, O.
  • Journal Title: Nucleic Acids Res. 37 Pages: 2910-2925
• [Journal Article] RNA helicase module in an acetyltransferase that modifies a specific tRNA anticodon (2009)
  • Author(s): Chimnaronk, S., Suzuki, T., Manita, T., Ikeuchi, Y., Yao, M., Suzuki, T., Tanaka, I.
  • Journal Title: EMBO J. 28 Pages: 1362-1373
• [Journal Article] Chemical synthesis and properties of 5-taurinomethyluridine and 5-taurinomethyl-2-thiouridine (2009)
  • Author(s): Ogata, T., Shimazaki, T., Umemoto, T., Kurata, S., Ohtsuki, T., Suzuki, T., Wada, T.
  • Journal Title: J.Org.Chem. 74 Pages: 2585-2588
• [Journal Article] Selective stabilization of mammalian microRNAs by 3'-adenylation mediated by the cytoplasmic poly(A) polymerase GLD-2 (2009)
  • Author(s): Katoh, T., Sakaguchi, Y., Miyauchi, K., Suzuki, T., Kashiwabara, S., Baba, T., Suzuki, T.
  • Journal Title: Genes Dev. 23 Pages: 433-438
• [Journal Article] Biogenesis and functions of thio-compounds in transfer RNA : comparison of bacterial and eukaryotic thiolation machineries (2009)
  • Author(s): Noma, A., Shigi, N., Suzuki, T.
  • Journal Title: DNA and RNA modification enzymes
• [Journal Article] Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions (2009)
  • Author(s): Noma, A., Sakaguchi, Y., Suzuki, T.
  • Journal Title: Nucleic Acids Res. 37 Pages: 1335-1352
• [Journal Article] Precise analysis of modification status at various stage of tRNA maturation in Saccharomyces cerevisiae (2009)
  • Author(s): Ohira, T., Miyauchi, K., Sakaguchi, Y., Suzuki, T., Suzuki, T.
  • Journal Title: Nucleic Acids Symp Ser (Oxf) 53 Pages: 301-302
• [Journal Article] Common Sulfur Transfer System for the Biosyntheses of Sulfur-containing tRNA and Cofactors (2008)
  • Author(s): Shigi, N., Sakaguchi, Y., Asai, S., Suzuki, T., Watanabe, K.
  • Journal Title: EMBO J. 27 Pages: 3267-3278
• [Journal Article] The RNA acetyltransferase driven by ATP hydrolysis synthesizes N4-acetylcytidine of tRNA anticodon (2008)
  • Author(s): Ikeuchi, Y., Kitahara, K., Suzuki, T.
  • Journal Title: EMBO J. 27 Pages: 2194-2203
• [Journal Article] Measuring mRNA decay in human mitochondria (2008)
  • Author(s): Nagao, A., Shigi-Hino, N., Suzuki, T.
  • Journal Title: Methods in Enzymol. 447 Pages: 489-499
• [Journal Article] Ribosomal RNAs are tolerant toward genetic insertions : Evolutionary origin of the expansion segments (2008)
  • Author(s): Yokoyama, T., Suzuki, T.
  • Journal Title: Nucleic Acids Res. 36 Pages: 3539-3551
• [Journal Article] Modified uridines with C5-methylene substituents at the first position of the tRNA anticodon stabilize U・G wobble pairing during decoding (2008)
  • Author(s): Kurata, S., Weixlbaumer, A., Ohtsuki, T., Shimazaki, T., Wada, T., Kirino, Y., Takai, K., Watanabe, K. Ramakrishnan, V., Suzuki, T.
  • Journal Title: J.Biol.Chem. 283 Pages: 18801-18811
• [Journal Article] Polyadenylation in mammalian mitochondria : Insights from recent studies (2008)
  • Author(s): Nagaike, T., Suzuki, T., Ueda, T.
  • Journal Title: Biochim Biophys Acta 1779 Pages: 266-269
• [Journal Article] Mechanism of mRNA deadenylation : evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases (2007)
  • Author(s): Funakoshi, Y., Doi, Y., Hosoda, N., Uchida, N., Osawa, M., Shimada, I., Tsujimoto, M., Suzuki, T., Katada, T., Hoshino, S.
  • Journal Title: Genes Dev. 21 Pages: 3135-3148
• [Journal Article] Wobble inosine tRNA modification is essential for cell cycle progression in G1/S and G2/M transitions in fission yeast (2007)
  • Author(s): Tsutsumi, S., Sugiura, R., Ma, Y., Tokuoka, H., Ohta, K., Ohte, R., Noma, A., Suzuki, T., Kuno, T.
  • Journal Title: J.Biol.Chem. 282 Pages: 33459-33465
• [Journal Article] Crystal structure of the radical SAM enzyme catalyzing tricyclic modified base formation in tRNA (2007)
  • Author(s): Suzuki, Y., Noma, A., Suzuki, T., Senda, M., Senda, T., Ishitani, R., Nureki, O
  • Journal Title: J.Mol.Biol. 372 Pages: 1204-1214
• [Journal Article] Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi-interacting RNAs at their 3' ends (2007)
  • Author(s): Saito, K., Sakaguchi, Y., Suzuki, T., Suzuki, T., Siomi, H., Siomi, M. C.
  • Journal Title: Genes Dev. 21 Pages: 1603-1608
• [Journal Article] Functional genetic selection of Helix 66 in Escherichia coli 23S rRNA identified the eukaryotic class of binding sequences for ribosomal protein L2 (2007)
  • Author(s): Kitahara, K., Kajiura, A., Sato, N. S., Suzuki, T.
  • Journal Title: Nucleic Acids Res. 35 Pages: 4018-4029
• [Journal Article] Mass spectrometric analysis of 3'-terminal nucleosides in non-coding RNAs (2007)
  • Author(s): Suzuki, T., Sakaguchi, Y., Suzuki, T.
  • Journal Title: Nat Protoc. 185 Pages: DOI : 10.1038/nprot.2007
• [Journal Article] Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S Ribosomal Subunit (2007)
  • Author(s): Dunham, C. M., Selmer, M., Phelps, S. S., Suzuki, T., Joseph, S., Ramakrishnan, V.
  • Journal Title: RNA 13 Pages: 817-823
• [Journal Article] The 3'-termini of mouse piwi-interacting RNAs are 2'-O-methylated (2007)
  • Author(s): Ohara, T., Sakaguchi, Y., Suzuki, T., Ueda, H., Miyauchi, K., Suzuki T.
  • Journal Title: Nat Struct Mol Biol. 14 Pages: 349-350
• [Journal Article] Thio modification of yeast cytosolic tRNA is an iron-sulfur protein-dependent pathway (2007)
  • Author(s): Nakai, Y., Nakai, M., Lill, R., Suzuki, T., Hayashi, H.
  • Journal Title: Mol Cell Biol. 27 Pages: 2841-2847
• [Journal Article] Chaplet column chromatography : isolation of a large set of individual RNAs in a single step. (2007)
  • Author(s): Suzuki, T., Suzuki, T.
  • Journal Title: Methods in Enzymol. 425 Pages: 231-239
• [Journal Article] Mass spectrometric identification and characterization of RNA-modifying enzymes (2007)
  • Author(s): Suzuki, T., Ikeuchi, Y., Noma, A., Suzuki, T., Sakaguchi, Y.
  • Journal Title: Methods in Enzymol. 425 Pages: 211-229
• [Journal Article] Automated parallel isolation of multiple species of non-coding RNAs by the reciprocal circulating chromatography method (2007)
  • Author(s): Miyauchi, K., Ohara, T., Suzuki, T.
  • Journal Title: Nucleic Acids Res. 35 Pages: e24
• [Journal Article] Specific residues at every third position of siRNA shape its efficient RNAi activity (2007)
  • Author(s): Katoh, T., Suzuki, T.
  • Journal Title: Nucleic Acids Res. 35 Pages: e27
• [Journal Article] Ligand-induced translation by the allosteric ribosome bearing an aptamer-fused rRNA (2007)
  • Author(s): Yokoyama, T., Suzuki, T.
  • Journal Title: Nucleic Acids Symp Ser (Oxf) 51 Pages: 383-384
• [Journal Article] Aminoacyl-tRNA surveillance by EF-Tu in mammalian mitochondria (2007)
  • Author(s): Nagao, A., Suzuki, T., Suzuki, T.
  • Journal Title: Nucleic Acids Symp Ser (Oxf) 51 Pages: 41-42
• [Journal Article] Animated Crystallography of Genetic Code Translation. (2007)
  • Author(s): Tomita, K., Numata, T., Fukai, T., Suzuki, T., Ishitani, R., Nureki, O.
  • Journal Title: Nucleic Acids Symp Ser (Oxf) 51 Pages: 101-102
• [Presentation] Biogenesis of Gin-mt tRNAG1n in human mitochondria (2010)
  • Author(s): Asuteka Nagao, Takeo Suzuki, Takayuki Katoh, Yuriko Sakaguchi, Tsutomu Suzuki
  • Organizer: The 23rd tRNA workshop
  • Place of Presentation: Aveiro
  • Year and Date: 20100128-20100202
• [Presentation] Novel wobble modification in tRNAIle responsible for decoding AUA codon in archaeal species ; convergent evolution of the decoding system across domains of life (2010)
  • Author(s): Satoshi Kimura, Yoshiho Ikeuchi, Tomoyuki Numata, Daigo Nakamura, Takashi Yokogawa, Kazuya Nishikawa, Takeshi Wada, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: The 23rd tRNA workshop
  • Place of Presentation: Aveiro
  • Year and Date: 20100128-20100202
• [Presentation] A landscape of tRNA modifications : complete chemical structures of total 48 species of Escherichia coli tRNAs determined by mass spectrometry (2010)
  • Author(s): Kenjyo Miyauchi, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: The 23rd tRNA workshop
  • Place of Presentation: Aveiro
  • Year and Date: 20100128-20100202
• [Presentation] Requirements for in vitro formation of 5-carboxymethylaminometyluridine at the wobble position in Escherichia coli tRNAs (2010)
  • Author(s): Takeo Suzuki, Tomoyuki Numata, Takuo Osawa, Tsutomu Suzuki
  • Organizer: The 23rd tRNA workshop
  • Place of Presentation: Aveiro
  • Year and Date: 20100128-20100202
• [Presentation] RNAの直接解析で見えてきたもの～microRNAが有する質的な情報と選択的安定化機構～ (2010)
  • Author(s): 鈴木勉
  • Organizer: 臨床応用を目指した最前線セミナー～microRNAを標的とした診断・治療の開発に向けて～
  • Place of Presentation: 東京
  • Year and Date: 2010-02-15
• [Presentation] A landscape of A-to-I RNA editing in human transcriptome : a hidden layer of gene expression produced by qualitative information embedded in RNA molecules (2009)
  • Author(s): Tsutomu Suzuki, Hiroki Ueda, Takanori Yano, Shunpei Okada, Hideki Terajima, Totai Mitsuyama, Atsushi Toyoda, Asao Fujiyama, Hitomi Kawabata, Masayuki Sakurai
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] マススペクトロメトリーによるmiRNAの直接プロファイリングとmiRNAの特異的安定化機構の発見 (2009)
  • Author(s): 坂口裕理子, 加藤敬行, 上田宏生, 宮内健常, 柏原真一, 馬場忠, 姜秉一, 栗原靖之, 鈴木健夫, 鈴木勉
  • Organizer: 第82回日本生化学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-20091024
• [Presentation] Precise analysis of modification status at various stage of tRNA maturation in Saccharomyces cerevisiae (2009)
  • Author(s): Takayuki Ohira, Kenjyo Miyauchi, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: 国際核酸化学シンポジウム
  • Place of Presentation: 富山
  • Year and Date: 20090927-20091001
• [Presentation] 出芽酵母tRNA前駆体の細胞質から核への逆行性の輸送が塩基修飾の形成に関わる (2009)
  • Author(s): 大平高之, 鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] RNAドメインの序列的な転写はリボソームの生合成に必須ではない (2009)
  • Author(s): 北原圭, 鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] イントロン領域におけるA-to-I RNAエディティングの機能解析 (2009)
  • Author(s): 矢野孝紀, 櫻井雅之, 上日宏生, 岡日俊平, 川畑瞳, 鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] 大腸菌RlmKLは23S rRNA中における2種類の異なるメチル化塩基の生合成を触媒する新しいタイプのRNA修飾酵素である (2009)
  • Author(s): Satoshi Kimura, Yoshiho Ikeuchi, Takeo Suzuki, Kei Kitahara, Yuriko Sakaguchi, Tsutomu Suzuki
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] 往復循環クロマトグラフィーを用いた微量RNAの全自動単離精製と修飾解析 (2009)
  • Author(s): 宮内健常, 坂口裕理子, 鈴木健夫, 鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] A-to-I RNA editing によるmiRNA依存的翻訳制御の調節 (2009)
  • Author(s): 矢野孝紀, 櫻井雅之, 上田宏生, 岡田俊平, 川畑瞳, 鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] ICE法と次世代シーケンサーを組み合わせたヒト成人脳由来RNA中に含まれるイノシン化部位の網羅的探索 (2009)
  • Author(s): 上田宏生, 櫻井雅之, 川畑瞳, 光山統泰, 矢野孝紀, 岡田俊平, 豊田敦, 藤山秋佐夫, 鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] ヒト転写産物におけるRNAエディティング部位探索と機能解析 (2009)
  • Author(s): 櫻井雅之, 川畑瞳, 矢野孝紀, 岡田俊平, 光山統泰, 豊田敦, 藤山秋佐夫, 上田宏生, 鈴木勉
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20090727-20090729
• [Presentation] Large scale identification of A-to-I editing sites in the human brain transcriptome by the ICE method ; implication for modulatory effect of A-to-I editing on translational repression mediated by miRNAs (2009)
  • Author(s): Masayuki Sakurai, Takanori Yano, Shunpei Okada, Hitomi Kawabata, Totai Mitsuyama, Hiroki Ueda, Tsutomu Suzuki
  • Organizer: Gordon Reseach Conference for RNA editing
  • Place of Presentation: Galveston
  • Year and Date: 20090112-20090116
• [Presentation] The acetylation of 18S rRNA is an essential modification to synthesize the small subunit of eukaryotic ribosome (2009)
  • Author(s): Satoshi Ito, Yu Akamatsu, Akiko Noma, Satoshi Kimura, Yoshiho Ikeuchi, Yoshikazu Tanaka, Kenjyo Miyauchi, Isao Tanaka, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Direct analysis of small non-coding RNAs by mass spectrometry (2009)
  • Author(s): Tsutomu Suzuki
  • Organizer: Joint Symposium of the 5th Annual Meeting of OTS and The 19th Antisense Symposium
  • Place of Presentation: 福岡
  • Year and Date: 2009-11-06
• [Presentation] トップランナーズレクチャー RNAの直接解析で見えてきたもの (2009)
  • Author(s): 鈴木勉
  • Organizer: 第82回日本生化学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] RNAの直接解析で見えてきたもの～microRNAが有する質的な情報と選択的安定化機構～ (2009)
  • Author(s): 鈴木勉
  • Organizer: KASTセミナー, 機能性RNAコース～機能性RNAの歴史と応用展開～
  • Place of Presentation: 神奈川
  • Year and Date: 2009-09-16
• [Presentation] Circular permutants of the ribosomes in the cell (2009)
  • Author(s): Kei Kitahara, Tsutomu Suzuki
  • Organizer: Experimental biology 2009
  • Place of Presentation: New Orleans
  • Year and Date: 2009-04-18
• [Presentation] 13th annual meeting of the RNA society (RNA2008) (2008)
  • Author(s): Takayuki Ohira, Yuki Takeuchi, Yuriko Sakaguchi, Hiroki Ueda, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: Mass spectrometric identification of non-coding RNAs in ribonucleoprotein complexes in yeast
  • Place of Presentation: Berlin
  • Year and Date: 20080728-20080803
• [Presentation] 酵母tRNAウォブル位のチオウリジン修飾はユビキチン様の反応機構を経由する (2008)
  • Author(s): 野間章子, 鈴木勉
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080723-20080725
• [Presentation] 往復循環クロマトグラフィー法による non-coding RNAの全自動単離精製 (2008)
  • Author(s): 宮内健常, 坂口裕理子, 鈴木健夫, 鈴木勉
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080723-20080725
• [Presentation] マイクロRNA前駆体に見出された Dicing 部位を規定する基質モチーフとmiRNAの3味端形成加藤敬行 (2008)
  • Author(s): 坂口裕理子, 宮内健常, 鈴木健夫, 鈴木勉
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080723-20080725
• [Presentation] 大腸菌23S rRNAの円順列変異の取得とリボソーム構築原理の新知見 (2008)
  • Author(s): 北原圭, 鈴木勉
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080723-20080725
• [Presentation] RNA mass spectrometry : a platform technology for non-coding RNA research (2008)
  • Author(s): Yuriko Sakaguchi, Hiroki Ueda, Takeo Suzuki, Takayuki Katoh, Takeshi Seguchi, Kenjyo Miyauchi, Tsutomu Suzuki
  • Organizer: 56th ASMS Conference on Mass Spectrometry
  • Place of Presentation: Denver
  • Year and Date: 20080601-20080605
• [Presentation] Mass spectrometric identification of non-coding RNAs in ribonucleoprotein complexes ; biochemical approach to RNA-protein interactome (2008)
  • Author(s): 鈴木勉
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学大会合同大会 (BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] RNA修飾の生合成と機能に関する研究 (Biogenesis and functions of RNA modifications) (2008)
  • Author(s): 鈴木勉
  • Organizer: 第6回 日本分子生物学会三菱化学奨励賞 受賞講演
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] RNAマススペクトロメトリー ―機能性RNAの直接解析で見えてきたもの― (2008)
  • Author(s): 鈴木勉
  • Organizer: 2008質量分析計 ユーザーズフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2008-10-22
• [Presentation] RNAマススペクトロメトリー ―機能性RNAの直接解析で見えてきたもの― (2008)
  • Author(s): 鈴木勉
  • Organizer: 発生工学・疾患モデル研究会「第70回定例会」
  • Place of Presentation: 東京
  • Year and Date: 2008-09-10
• [Presentation] 自分らしいRNA研究を追い求めてきて (2008)
  • Author(s): 鈴木勉
  • Organizer: RNAフロンティアミーティング2008
  • Place of Presentation: 京都
  • Year and Date: 2008-06-04
• [Presentation] 東工大生命理工GCOEシンポジウム「ようこそ先輩」 (2008)
  • Author(s): 鈴木勉
  • Organizer: RNAに魅せられて-企業での経験をアカデミックな研究に生かす
  • Place of Presentation: 長津田
  • Year and Date: 2008-01-28
• [Presentation] Small non-coding RNAの直接解析で見えてきたもの (2007)
  • Author(s): 鈴木勉
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学大会合同大会 (BMB2007)
  • Place of Presentation: 横浜
  • Year and Date: 20071211-20071215
• [Presentation] Aminoacyl-tRNA surveillance by EF-Tu in mammalian mitochondria (2007)
  • Author(s): Asuteka Nagao, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: 第5回国際核酸化学シンポジウム (NACS2007)
  • Place of Presentation: 東京
  • Year and Date: 20071120-20071122
• [Presentation] Ligand-induced translation by the allosteric ribosome bearing an aptamer-fused rRNA (2007)
  • Author(s): Takeshi Yokoyama, Tsutomu Suzuki
  • Organizer: 第5回国際核酸化学シンポジウム (NACS2007)
  • Place of Presentation: 東京
  • Year and Date: 20071120-20071122
• [Presentation] Quality control of aminoacyl-tRNAs by kinetic competition of aminoacyltRNA synthetases and EF-Tu surveillance in mammalian mitochondria (2007)
  • Author(s): Asuteka Nagao, Takeo Suzuki, Tsutomu Suzuki
  • Organizer: 22nd International tRNA Workshop
  • Place of Presentation: ウプサラ
  • Year and Date: 20071101-20071106
• [Presentation] Genome-wide identification of genes responsible for 2-thiolation of 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) at wobble position of yeast tRNAs (2007)
  • Author(s): Akiko Noma, Tsutomu Suzuki
  • Organizer: 22nd International tRNA Workshop
  • Place of Presentation: ウプサラ
  • Year and Date: 20071101-20071106
• [Presentation] TmcA catalyzes 4-acetylcytidine formation at wobble position of bacterial tRNAMet (2007)
  • Author(s): Yoshiho Ikeuchi, Sarin Chimnaronk, Min Yao, Isao Tanaka, Tsutomu Suzuki
  • Organizer: 22nd International tRNA Workshop
  • Place of Presentation: ウプサラ
  • Year and Date: 20071101-20071106
• [Presentation] Mass spectrometric characterization of small non-coding RNAs (2007)
  • Author(s): Tsutomu Suzuki
  • Organizer: Yonsei University-The University of Tokyo Joint Symposium
  • Place of Presentation: ソウル
  • Year and Date: 20070807-20070809
• [Presentation] 精子形成における哺乳類piRNAメチル化酵素の機能解析 (2007)
  • Author(s): 長岡真, 大原智也, 中川真一, 加藤敬行, 五島直樹, 野村信夫, 鈴木健夫, 坂口裕理子, 鈴木勉
  • Organizer: 第9回日本RNA学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 20070728-20070731
• [Presentation] RNA mass spectrometry : a platform technology for non-coding RNA research (2007)
  • Author(s): 鈴木勉
  • Organizer: 日本ヒトプロテオーム機構 (JHUPO) 第5回大会
  • Place of Presentation: 東京
  • Year and Date: 2007-07-31
• [Presentation] RNAマススペクトロメトリー (2007)
  • Author(s): 鈴木勉
  • Organizer: 日本プロテオーム機構 (JHUPO) 第5回大会
  • Place of Presentation: 東京
  • Year and Date: 2007-07-31
• [Presentation] RNA修飾が関与する生命現象へのアプローチ (2007)
  • Author(s): 鈴木勉
  • Organizer: RNA分子のダイナミズム-生命現象の根幹をなす機能性RNA
  • Place of Presentation: 名古屋
  • Year and Date: 2007-07-28
• [Presentation] Ribosome 2007 : Form and Function Cape Cod, USA MECHANISTIC AND ARCHITECTURAL ANALYSIS OF E. COLI RIBOSOMAL RNAS USING THE COMPREHENSIVE GENETIC SELECTION (2007)
  • Author(s): Neuza S. Sato, Kei Kitahara, Takeshi Yokoyama, Naomi Hirabayashi, Taeko Komoda, Steven S. Phelps, Simpson Joseph, Rajendra K. Agrawal, Ilana Agmon, Ada Yonath, Tsutomu Suzuki
  • Year and Date: 2007-06-05
• [Presentation] Mass spectrometric characterization of small non-coding RNAs ; identification of 2'-O-methylation at the 3'-termini of mouse piwi-interacting RNAs (2007)
  • Author(s): Tsutomu Suzuki, Tomoya Ohara, Takeo Suzuki, Hiroki Ueda, Takeshi Seguchi, Kenjyo Miyauchi, Yuriko Sakaguchi
  • Organizer: RNA 2007 : 12th Annual Meeting of the RNA Society
  • Place of Presentation: Madison
  • Year and Date: 2007-06-01
• [Presentation] RNA mass spectrometry reveals qualitative aspects of non-coding RNAs (2007)
  • Author(s): 鈴木勉
  • Organizer: 細胞生物学会/発生生物学会シンポジウム「Frontiers in RNA Biology」
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-30
• [Presentation] RNA修飾の多彩な機能と生命現象 (2007)
  • Author(s): 鈴木勉
  • Organizer: お茶の水がん学アカデミア第34回集会
  • Place of Presentation: 東京
  • Year and Date: 2007-04-24
• [Presentation] 機能性RNAのマススペクトロメトリー～見過ごされているRNAの質的な情報と高次生命現象へのアプローチ～ (2007)
  • Author(s): 鈴木勉
  • Organizer: 化学とバイオの架け橋
  • Place of Presentation: 本郷
  • Year and Date: 2007-04-07
• [Book] ミトコンドリア脳筋症とtRNAのタウリン修飾欠損, 細胞工学 (2010)
  • Author(s): 鈴木勉, 鈴木健夫
  • Total Pages: 137-143
• [Book] マススペクトロメトリーを用いたmiRNAの直接プロファイリング, 分子細胞治療 (2009)
  • Author(s): 坂口裕理子, 鈴木勉
  • Total Pages: 36-41
• [Book] 細胞質に局在するGLD-2による3'末端アデニル化とmiRNAの選択的安定化機構, 実験医学 (2009)
  • Author(s): 加藤敬行, 鈴木勉
  • Total Pages: 1746-1750
• [Book] mRNAの塩基修飾・編集と遺伝子発現制御機構 (2009)
  • Author(s): 櫻井雅之, 矢野孝紀, 岡日俊平, 竹内祐樹, 鈴木勉
  • Total Pages: 2086-2091
  • Publisher: 共立出版
• [Book] 核酸アナログとsiRNAの配列設計 (2009)
  • Author(s): 加藤敬行, 宮内健常, 鈴木勉
  • Publisher: シーエムシー出版
• [Book] 転写後修飾によるRNA機能制御 (2009)
  • Author(s): 櫻井雅之, 鈴木勉
  • Total Pages: 149-155
  • Publisher: 秀潤社
• [Book] 大腸菌からのRNA抽出 (2008)
  • Author(s): 宮内健常, 鈴木勉
  • Total Pages: 17-20
  • Publisher: 羊土社
• [Book] 質量分析法を用いたRNA修飾ヌクレオシドの解析 (2008)
  • Author(s): 鈴木健夫, 鈴木勉
  • Total Pages: 177-181
  • Publisher: 羊土社
• [Book] 有効なsiRNAのデザインと細胞への導入 (2008)
  • Author(s): 加藤敬行, 鈴木勉
  • Total Pages: 99-102
  • Publisher: 羊土社
• [Book] 「RNAとゲノム (最近の話題)」-座談会 機能性RNA研究の展望-, ゲノム医学 (2007)
  • Author(s): 渡辺公綱, 浅井潔, 鈴木勉, 廣瀬哲郎
  • Total Pages: 79-84
• [Remarks] ホームページ等 siExplorer : effective siRNA design algorithm.
  • URL: http://rna.chem.t.u-tokyo.ac.jp/cgi/siexplorer.htm
• [Patent(Industrial Property Rights)] イソロイシンtRNA(tRNAIle)のライシジン合成酵素(TilS)としてのmesJ遺伝子産物及びその相同性遺伝子 (COG0037) (2010)
  • Inventor(s): 鈴木勉
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 特許 特願2003-329762
  • Acquisition Date: 2010-04-09
• [Patent(Industrial Property Rights)] RNA中のイノシン化部位の検出方法 (2005)
  • Inventor(s): 鈴木勉, 櫻井雅之
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 特許 特願2005-229335, PCT/JP2006/315581
  • Filing Date: 2005-08-08
• [Patent(Industrial Property Rights)] 往復循環クロマトグラフィーを用いた生体高分子の単離方法 (2005)
  • Inventor(s): 鈴木勉, 宮内健常
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 特許 特願2005-224992, PCT/JP2006/315271
  • Filing Date: 2005-08-03