Improvement of nitrogen-use-efficiency based on molecular mechanisms of ammonium assimilation and nitrogen recycling system in rice

2009 Fiscal Year Self-evaluation Report

• Project/Area Number: 19208007
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Tohoku University
• Principal Investigator: YAMAYA Tomoyuki Tohoku University, 大学院・農学研究科, 教授 (30144778)
• Project Period (FY): 2007 – 2009
• Keywords: イネ / アンモニア同化 / 窒素リサイクル機構 / 窒素利用効率 / 遺伝子破壊 / サイトゾル型グルタミン合成酵素 / NADH-グルタミン酸合成酵素

Research Abstract

本研究では、イネのゲノム解析完了に伴って新規に見いだされたサイトゾル型グルタミン合成酵素(GS1;3)とグルタミン酸合成酵素(NADH-GOGAT2)の機能解明と、窒素利用効率向上に関わる統合的な分子基盤を構築することを目的としている。主に、遺伝子破壊変異体の活用と、窒素利用効率の制御に関わる新規遺伝子の探索と機能を明らかにする計画である。

Research Products

• [Journal Article] Fine-mapping of qRL6.1, a major QTL for root length of rice seedlings grown under a wide range of NH_4^+ concentration in hydroponic conditions (2010)
  • Author(s): Obara, M., Tamura, W., Ebitani, T., Yano, M., Sato, T., Yamaya, T.
  • Journal Title: Theoretical Applied Genetics (in press)
  • Peer Reviewed
• [Journal Article] Reverse genetics approach to characterize a function of NADH-glutamate synthasel in rice plants (2010)
  • Author(s): Tamura, W., Hidaka, Y., Tabuchi, M., Kojima, S., Hayakawa, T., Sato, T., Obara, M., Kojima, M., Sakakibara, H., Yamaya, T.
  • Journal Title: Amino Acids (in press)
  • Peer Reviewed
• [Journal Article] Pleiotropic modulation of carbon and nitrogen metabolism in Arabidopsis plants overexpressing NAD kinase 2 gene (2009)
  • Author(s): Takahashi, H., Takahara, K., Hashida, S., Hirabayashi, T., Fujimori, T., Kawai-Yamada, M., Yamaya, T., Yanagisawa, S., Uchimiya, H.
  • Journal Title: Plant Physiol 151 Pages: 100-113
  • Peer Reviewed
• [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice (Oryza sativa L.) (2008)
  • Author(s): Kudo, T., Kawai, A., Yamaya, T., Hayakawa, T.
  • Journal Title: Physiol. Plant. 133 Pages: 167-179
  • Peer Reviewed
• [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis (2008)
  • Author(s): Sugawara, H., Ueda, N., Kojima, M., Makita, N., Yamaya, T., Sakakibara, H.
  • Journal Title: Proc.Natl.Acad.Sci.USA 105 Pages: 2734-2739
  • Peer Reviewed
• [Journal Article] Increased Rubisco content in transgenic rice transfbrmed with "sense" rbcS gene (2007)
  • Author(s): Suzuki, Y., Ohkubo, M., Hatakeyama, H., Ohashi, K., Yoshizawa, R., Kojima, S., Hayakawa, T., Yamaya, T., Mae, T., Makino, A.
  • Journal Title: Plant Cell Physiol 48 Pages: 626-637
  • Peer Reviewed
• [Journal Article] Assimilation of ammonium-ions and re-utilization of nitrogen in rice (Oryza sativa L) (2007)
  • Author(s): Tabuchi, M., Abiko, T., Yamaya, T.
  • Journal Title: J.Exp.Bot. 58 Pages: 2319-2327
  • Peer Reviewed
• [Presentation] イネの窒素飢餓ストレス (2009)
  • Author(s): 山谷知行
  • Organizer: 日本土壌肥料学会2009年度京都大会シンポジウム「植物のミネラルストレス応答」
  • Place of Presentation: 京都 (招待講演)
  • Year and Date: 20090915-20090917
• [Presentation] Function of glutamine synthetase and glutamate synthase in rice plants. 11^<th> International Congress on Amino Acids (2009)
  • Author(s): Yamaya, T.
  • Organizer: Peptides and Proteins
  • Place of Presentation: Vienna, Austria (招待講演)
  • Year and Date: 20090803-20090807
• [Presentation] Molecular mechanisms for plant biomass production-rice as a model plant (2008)
  • Author(s): Yamaya, T.
  • Organizer: UCR Tech Horizon 2008 Conference
  • Place of Presentation: UC Riverside, CA, USA (招待講演)
  • Year and Date: 20080513-20080514
• [Presentation] Genetic and physiological approach of nitrogen utilization toward improving environmental adaptation of rice. Workshop on Development of Environmentally-friendly (2008)
  • Author(s): Obara, M., Yamaya, T.
  • Organizer: Water-saving Technologies for Rice
  • Place of Presentation: Tsukuba, Japan (招待講演)
  • Year and Date: 2008-07-18
• [Presentation] Assimilation of ammonium ions and re-utilization of nitrogen in rice (2007)
  • Author(s): Yamaya, T.
  • Organizer: Nitrogen2007-An International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Lancaster University, UK (招待講演)
  • Year and Date: 20070727-20070731
• [Book] India, Ammonia absorption and assimilation in plants, In : "Nitrogen Assinlilation in Plants" (2010)
  • Author(s): Kojima, K., von Wiren, N., Yamaya, T.
  • Publisher: Research Signpost 社(印刷中)
• [Book] 細胞内アンモニア代謝、アンモニアトランスポーター、「植物栄養学 第2版」間藤徹, 馬建鋒, 藤原徹, 編 (2010)
  • Author(s): 山谷知行
  • Publisher: 文永堂出版(印刷中)
• [Book] b窒素代謝、「植物の百科事典」 (2009)
  • Author(s): 山谷知行
  • Total Pages: 16
  • Publisher: 朝倉書店
• [Book] 1. 2物質代謝、「植物の百科事典」 (2009)
  • Author(s): 山谷知行
  • Total Pages: 13-14
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/cellbio/index-j.htm